Sun H, Pu J, Chen F, Wang J, Han Z
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Insect Mol Biol. 2017 Jun;26(3):343-355. doi: 10.1111/imb.12299. Epub 2017 Mar 16.
ATP-binding cassette (ABC) transporters are membrane-bound proteins involved in the movement of various substrates, including drugs and insecticides, across the lipid membrane. Demonstration of the role of human ABC transporters in multidrug resistance has led to speculation that they might be an important mechanism controlling the fate of insecticides in insects. However, the role of ABC transporters in insects remains largely unknown. The small brown planthopper, Laodelphax striatellus Fallén, has developed resistance to most of the insecticides used for its control. Our goals were to identify the ABC transporters in La. striatellus and to examine their involvement in resistance mechanisms, using related strains resistant to chlorpyrifos, deltamethrin and imidacloprid, compared with the susceptible strain. Based on the transcriptome of La. striatellus, 40 full-length ABC transporters belonging to the ABCA-ABCH subfamilies were identified. Quantitative PCR revealed that over 20% of genes were significantly up-regulated in different resistant strains, and eight genes from the ABCB/C/D/G subfamilies were up-regulated in all three resistant strains, compared with the susceptible strain. Furthermore, synergism studies showed verapamil significantly enhanced insecticide toxicity in various resistant strains but not in the susceptible strain. These results suggest that ABC transporters might be involved in resistance to multiple insecticides in La. striatellus.
ATP结合盒（ABC）转运蛋白是一类膜结合蛋白，参与包括药物和杀虫剂在内的各种底物跨脂质膜的转运。人类ABC转运蛋白在多药耐药性中的作用已得到证实，这引发了一种推测，即它们可能是控制昆虫体内杀虫剂命运的重要机制。然而，ABC转运蛋白在昆虫中的作用在很大程度上仍不清楚。灰飞虱（Laodelphax striatellus Fallén）已对大多数用于防治它的杀虫剂产生了抗性。我们的目标是鉴定灰飞虱中的ABC转运蛋白，并与敏感品系相比，利用对毒死蜱、溴氰菊酯和吡虫啉具有抗性的相关品系，研究它们在抗性机制中的作用。基于灰飞虱的转录组，鉴定出了40个属于ABCA - ABCH亚家族的全长ABC转运蛋白。定量PCR结果显示，超过20%的基因在不同抗性品系中显著上调，与敏感品系相比，ABCB/C/D/G亚家族的8个基因在所有三个抗性品系中均上调。此外，增效研究表明，维拉帕米显著增强了各种抗性品系中的杀虫剂毒性，但对敏感品系没有影响。这些结果表明，ABC转运蛋白可能参与了灰飞虱对多种杀虫剂的抗性。
