Department of Bioengineering, Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.
Peoples Friendship University of Russia (RUDN University), Moscow, 117198, Russia.
Microb Ecol. 2020 May;79(4):785-800. doi: 10.1007/s00248-019-01437-0. Epub 2019 Nov 1.
Haematococcus lacustris is a biotechnologically important green unicellular alga producing widely used keta-karotenoid astaxanthin. In natural habitats, it exists in the form of algal-bacterial community, and under laboratory conditions, it is also accompanied by bacteria. The issue of the bacterial composition of industrial algal cultures is widely recognized as important. However, there is a dearth of information about bacterial composition of H. lacustris communities. In current work, we analyze the composition of natural H. lacustris communities from the White Sea coastal temporal rock ponds. For the first time, a 16S rRNA gene-based metagenome of natural H. lacustris bacterial communities has been generated. Main results of its analysis are as follow. Bacterial families Comamonadaceae, Cytophagaceae, Xanthomonadaceae, Acetobacteraceae, Rhodobacteraceae, and Rhodocyclaceae were observed in all studied H. lacustris natural communities. They also contained genera Hydrogenophaga and Cytophaga. Bacteria from the Hydrogenophaga genus were present in H. lacustris cultures after their isolation under the conditions of laboratory cultivation. Similar to other planktonic microalgae, H. lacustris forms a phycosphere around the cells. In this zone, bacteria attached to the algal surface. The contact between H. lacustris and bacteria is maintained even after sample drying. The study provides information about possible members of H. lacustris core microbiome, which can be presented in the industrial and laboratory cultures of the microalga.
盐泽杜氏藻是一种生物技术上重要的产广泛应用的酮式类胡萝卜素虾青素的绿色单细胞藻类。在自然生境中，它以藻类-细菌群落的形式存在，而在实验室条件下，它也伴随着细菌。工业藻类培养物的细菌组成问题被广泛认为是重要的。然而，关于盐泽杜氏藻群落的细菌组成的信息却很少。在当前的工作中，我们分析了来自白海沿海季节性岩石池塘的天然盐泽杜氏藻群落的组成。我们首次生成了基于 16S rRNA 基因的天然盐泽杜氏藻细菌群落的宏基因组。其分析的主要结果如下。在所有研究的天然盐泽杜氏藻自然群落中都观察到了根瘤菌科、噬纤维菌科、黄单胞菌科、醋杆菌科、红杆菌科和红环菌科。它们还含有氢单胞菌属和噬纤维菌属。在实验室培养条件下分离盐泽杜氏藻后，该属的细菌存在于盐泽杜氏藻培养物中。与其他浮游微藻类似，盐泽杜氏藻在细胞周围形成藻球。在这个区域，细菌附着在藻类表面。即使在样品干燥后，盐泽杜氏藻和细菌之间的接触也能保持。该研究提供了关于盐泽杜氏藻核心微生物组的可能成员的信息，这些信息可以在微藻的工业和实验室培养中呈现。
