Selivanova Elena A, Yakimov Michail M, Kataev Vladimir Y, Khlopko Yuri A, Balkin Alexander S, Plotnikov Andrey O
Institute for Cellular and Intracellular Symbiosis of the Ural Branch of Russian Academy of Sciences, Orenburg Federal Research Center of the Ural Branch of Russian Academy of Sciences, 460000 Orenburg, Russia.
Extreme Microbiology, Biotechnology and Astrobiology Group, Institute of Polar Research, The Institute of Polar Sciences of the National Research Council (ISP-CNR), 98122 Messina, Italy.
Microorganisms. 2024 Sep 26;12(10):1947. doi: 10.3390/microorganisms12101947.
The influence of microalgae on the formation of associated prokaryotic assemblages in halophilic microbial communities is currently underestimated. The aim of this study was to characterize shifts in prokaryotic assemblages of halophilic microalgae upon their transition to laboratory cultivation. Monoalgal cultures belonging to the classes Chlorodendrophyceae, Bacillariophyceae, Trebouxiophyceae, and Chlorophyceae were isolated from habitats with intermediate salinity, about 100 g/L, nearby Elton Lake (Russia). Significant changes were revealed in the structure of algae-associated prokaryotic assemblages, indicating that microalgae supported sufficiently diverse and even communities of prokaryotes. Despite some similarities in their prokaryotic assemblages, taxon-specific complexes of dominant genera were identified for each microalga species. These complexes were most different among , likely due to their close association with microalgae. Other taxon-specific bacteria included members of phylum ( in assemblages of sp.) and class in microbiomes of ). After numerous washings of algal cells, only alphaproteobacteria remained in all assemblages of , likely due to a firm attachment to the microalgae cells. Our results may be useful for further efforts to develop technologies applied for industrial cultivation of halophilic microalgae and for developing approaches to obtain new prokaryotes with a microalgae-associated lifestyle.
目前，微藻对嗜盐微生物群落中相关原核生物组合形成的影响被低估了。本研究的目的是描述嗜盐微藻向实验室培养过渡过程中原核生物组合的变化。从俄罗斯叶尔顿湖附近盐度约为100 g/L的中度盐度栖息地分离出属于绿枝藻纲、硅藻纲、小球藻纲和绿藻纲的单藻培养物。研究发现，与藻类相关的原核生物组合结构发生了显著变化，这表明微藻支持了足够多样甚至丰富的原核生物群落。尽管它们的原核生物组合存在一些相似之处，但每种微藻物种都鉴定出了特定分类群的优势属复合体。这些复合体在……之间差异最大，可能是由于它们与微藻的密切关联。其他特定分类群的细菌包括……门的成员（在……属的组合中）和……纲的成员（在……的微生物群落中）。在对藻类细胞进行多次洗涤后，所有……组合中仅剩下α-变形菌，这可能是因为它们牢固地附着在微藻细胞上。我们的研究结果可能有助于进一步努力开发适用于嗜盐微藻工业培养的技术，并有助于开发获得具有与微藻相关生活方式的新原核生物的方法。
