School of Food Science and Biotechnology, Institute of Agricultural Science and Technology, Kyungpook National University, Daegu, South Korea.
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, United States.
Front Cell Infect Microbiol. 2019 Oct 17;9:355. doi: 10.3389/fcimb.2019.00355. eCollection 2019.
The Mkt1-Pbp1 complex promotes mating-type switching by regulating the translation of mRNA in . Here, we performed immunoprecipitation assays and mass spectrometry analyses in the human fungal pathogen to show that Pbp1, a poly(A)-binding protein-binding protein, interacts with Mkt1 containing a PIN like-domain. Association of Pbp1 with Mkt1 was confirmed by co-immunoprecipitation assays. Results of spot dilution growth assays showed that unlike deletion mutant strains, deletion mutant strains were not resistant to heat stress compared with wild-type. However, similar to the deletion mutant strains, the deletion mutants exhibited both, defective dikaryotic hyphal production and reduced pheromone gene (α1) expression during mating. In addition, deletion of caused attenuated virulence in a murine intranasal inhalation model. Taken together, our findings reveal that Mkt1 plays a crucial role in sexual reproduction and virulence in .
Mkt1-Pbp1 复合物通过调节 mRNA 的翻译促进交配型转换。在这里，我们在人类真菌病原体 中进行了 免疫沉淀测定和质谱分析，结果表明 Pbp1 是一种多（A）结合蛋白结合蛋白，与含有 PIN 样结构域的 Mkt1 相互作用。Pbp1 与 Mkt1 的关联通过共免疫沉淀测定得到了证实。斑点稀释生长测定的结果表明，与野生型相比，与 缺失突变体菌株相比， 缺失突变体菌株对热应激没有抗性。然而，与 缺失突变体菌株类似， 缺失突变体表现出双核菌丝产生缺陷和交配过程中信息素基因（α1）表达减少。此外， 缺失导致在小鼠鼻内吸入模型中的毒力减弱。总之，我们的研究结果表明，Mkt1 在 中的有性生殖和毒力中起着至关重要的作用。
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