Olofinnade Anthony T, Adeyeba Adegboyega, Onaolapo Adejoke Y, Onaolapo Olakunle J
Department of Pharmacology, Therapeutics and Toxicology, Faculty of Basic Clinical Sciences, College of Medicine, Lagos State University, Ikeja, Lagos State, Nigeria.
Department of Pharmacology and Therapeutics, Ladoke Akintola University of Technology, Osogbo, Osun State, Nigeria.
Cent Nerv Syst Agents Med Chem. 2020;20(1):49-57. doi: 10.2174/1871524919666191104154009.
Azodicarbonamide is a dough-enhancer used in the process of breadmaking in countries like Nigeria. While there have been suggestions that it is a sensitizer of the respiratory system, there is a dearth of information on its effects on the central nervous system.
This study assessed the effects of azodicarbonamide on the central nervous system (ADA) in rats.
The effects of ADA-containing diet on neurobehaviour, brain antioxidant status, and neuromorphology of selected brain regions in rats were examined.
Forty adult rats were randomly-assigned into four groups of ten rats each, and were given standard diet or diet containing ADA at 1, 2 and 4% respectively. Rats were fed a standard diet or ADA-containing diet for a period of 28 days. Weekly body weight assessment and daily estimation of food intake were done. Behavioural tests {in the Open field, Y-maze, radial-arm maze, and Elevated Plus Maze (EPM)} were conducted on day 29. Twenty-four hours after the last behavioural test, animals were euthanised, whole brains were dissected, weighed, and either homogenised for assessment of lipid peroxidation and antioxidant status; or sectioned and processed for general histology.
Consumption of ADA-containing diet was associated with a significant decrease in weight gain/food intake, and significant suppression of horizontal locomotion and rearing behaviours; however, grooming activity increased significantly. Also, there was a significant reduction of open-arm time in the EPM and a significant increase in Y-maze alternation (at the lowest concentration of ADA). ADA-containing diet was not associated with significant changes in brain oxidative status or neuromorphology.
The study showed that while ADA-containing diet may alter neurobehaviour in rats; this was not associated with evidence of brain oxidative stress or neuro-histomorphological alterations.
偶氮二甲酰胺是一种在尼日利亚等国家用于面包制作过程的面团改良剂。虽然有人认为它是呼吸系统的致敏剂，但关于其对中枢神经系统影响的信息却很匮乏。
本研究评估了偶氮二甲酰胺对大鼠中枢神经系统（ADA）的影响。
研究了含ADA饮食对大鼠神经行为、脑抗氧化状态以及选定脑区神经形态的影响。
将40只成年大鼠随机分为四组，每组10只，分别给予标准饮食或分别含1%、2%和4% ADA的饮食。大鼠喂食标准饮食或含ADA饮食28天。每周进行体重评估，每天估计食物摄入量。在第29天进行行为测试{旷场试验、Y迷宫试验、放射状臂迷宫试验和高架十字迷宫试验（EPM）}。在最后一次行为测试24小时后，对动物实施安乐死，解剖全脑，称重，然后要么匀浆以评估脂质过氧化和抗氧化状态；要么切片并进行常规组织学处理。
食用含ADA饮食与体重增加/食物摄入量显著降低以及水平运动和竖毛行为受到显著抑制有关；然而，梳理活动显著增加。此外，在EPM中，开放臂停留时间显著减少，在Y迷宫试验中交替次数显著增加（在最低ADA浓度时）。含ADA饮食与脑氧化状态或神经形态的显著变化无关。
该研究表明，虽然含ADA饮食可能会改变大鼠的神经行为；但这与脑氧化应激或神经组织形态学改变的证据无关。
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