Onaolapo A Y, Adebayo A A, Onaolapo O J
Department of Anatomy, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Department of Pharmacology, Ladoke Akintola University of Technology, Osogbo, Osun State, Nigeria.
Pathophysiology. 2018 Mar;25(1):31-39. doi: 10.1016/j.pathophys.2017.12.001. Epub 2017 Dec 5.
In the current study, effects of oral phenytoin on hair growth in cyclophosphamide-treated rats were assessed with the goal of evaluating the ability of phenytoin to suppress chemotherapy-induced hair loss. Thirty-six rats were randomly assigned to six groups (1k6) of six each (n=6). In all groups, anagen was induced in flank skin of rats by depilation. On day 9 (anagen VI), rats were injected once with either distilled water (groups 1-3) or cyclophoshamide (groups 4-6). From day 10, rats in group 1 and 4 received oral vehicle (distilled water), groups 2 and 5 received oral phenytoin (50mg/kg), while groups 3 and 6 also received oral phenytoin (100mg/kg). Drug or vehicle was administered daily for a period of 28days. The flank area was serially photographed. At the end of the experimental period, rats were sacrificed to correlate visible hair growth with a histological profile of follicle response and recovery. Glutathione (GSH), glutathione peroxidase (GPX) and lipid peroxidation status were assessed. Cyclophosphamide (CYP) treatment was associated with gross morphologic and histological evidence of hair loss in the flanks, microscopic evidence of hair-shaft thinning, increased skin lipid peroxidation, decreased GSH level, and reduction in GPX activities. Phenytoin co-administration was associated with evidence of improved hair growth, increased hair-shaft thickness, reduced skin lipid peroxidation, increased GSH level, and increased GPX activities. This study showed that oral phenytoin can suppress hair loss due to CYP therapy in rats; however, further studies are needed to evaluate its potential application in chemotherapy-induced alopecia.
在本研究中，评估了口服苯妥英对环磷酰胺处理的大鼠毛发生长的影响，目的是评估苯妥英抑制化疗引起的脱发的能力。36只大鼠被随机分为6组（每组6只，n = 6）。所有组均通过拔毛诱导大鼠胁腹皮肤进入生长期。在第9天（生长期VI），大鼠一次性注射蒸馏水（第1 - 3组）或环磷酰胺（第4 - 6组）。从第10天开始，第1组和第4组大鼠口服赋形剂（蒸馏水），第2组和第5组大鼠口服苯妥英（50mg/kg），而第3组和第6组大鼠也口服苯妥英（100mg/kg）。每天给药或给予赋形剂，持续28天。对胁腹区域进行连续拍照。在实验期结束时，处死大鼠以将可见的毛发生长与毛囊反应和恢复的组织学特征相关联。评估了谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPX）和脂质过氧化状态。环磷酰胺（CYP）处理与胁腹脱发的大体形态学和组织学证据、毛干变细的显微镜证据、皮肤脂质过氧化增加、GSH水平降低以及GPX活性降低有关。联合使用苯妥英与毛发生长改善、毛干厚度增加、皮肤脂质过氧化减少、GSH水平增加以及GPX活性增加的证据有关。本研究表明，口服苯妥英可抑制大鼠因CYP治疗引起的脱发；然而，需要进一步研究以评估其在化疗引起的脱发中的潜在应用。
