Zhang Lihong, Zhuang Zhihong, Sun Yanan, Ma Shuhua, Yang Weifeng, Lei Hongtao, Ouyang Jingfeng, Wang Yi, Zuo Junling
Graduate School, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Morphological Laboratory, Expeimental Research Center, Research and Development Service Base, China Academy of Chinese Medical Sciences, Beijing 100700, China.
J Tradit Chin Med. 2017 Jun;37(3):308-313.
To investigate the neural differentiation capacity of water extraction of velvet antler.
Velvet antler (Cervus Nippon Temminck) polypeptide (VAP) was used to differentiate neural stem cells (NSCs) towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immunfluorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.
The differentiating cells look like neuron, some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron-like cells can express the special neural molecules.
Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide.
研究鹿茸水提取物的神经分化能力。
本研究采用鹿茸（梅花鹿）多肽（VAP）诱导神经干细胞（NSCs）向神经元分化。首先，通过水提取法获得VAP多肽。其次，观察细胞形态，采用酶联免疫吸附测定法检测培养基中的因子，并通过免疫荧光染色检测特异性神经分子。NSCs接种于细胞爬片上。神经元分化后，采用免疫荧光技术对分化的NSCs进行免疫细胞化学封片。
分化细胞形似神经元，培养基中可检测到一些特异性因子，如胶质细胞源性神经营养因子、神经生长因子，而分化的神经元样细胞可表达特异性神经分子。
鹿茸多肽可诱导NSCs向神经元分化。
