Department of Hand-Foot Microsurgery, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, 518052, People's Republic of China.
Department of Gynaecology, Shenzhen Nanshan District Maternal and Chlid Health Care Hospital, Shenzhen, 518000, People's Republic of China.
J Mol Histol. 2022 Dec;53(6):915-923. doi: 10.1007/s10735-022-10099-1. Epub 2022 Aug 29.
Functionalizing biomaterial substrates with biological signals shows promise in regulating cell behaviors through mimicking cellular microenvironment. Calcium phosphate (CaP) coating is an excellent carrier for immobilizing biological molecules due to its non-toxicity, good biocompatibility, biodegradability, and favorable affinity to plenty of molecules. In this study, we reported the adhesion, the viability and proliferation behaviors after oxidative stress injury of Schwann cells RSC96 on CaP immobilized with the Velvet Antler Peptide (VAP) isolated from velvet antler through coprecipitation process in modified Dulbecco's phosphate-buffered saline (DPBS) containing VAP. This approach provided well retention of functional molecules up to 28 days, and supported the adhesion and proliferation of RSC96 after oxidative stress injury without cytotoxicity. The simple and reproducible method of coprecipitation suggests that CaP is an ideal carrier to functionalize materials with biological molecules for peripheral nerve repair-related applications.
通过模拟细胞微环境，在生物材料基质上功能化生物信号显示出调节细胞行为的潜力。磷酸钙 (CaP) 涂层由于其无毒、良好的生物相容性、可生物降解性和对大量分子的良好亲和力，是固定生物分子的极好载体。在这项研究中，我们通过共沉淀过程在含有 Velvet Antler 肽 (VAP) 的改良 Dulbecco 磷酸盐缓冲盐水 (DPBS) 中报道了 Schwann 细胞 RSC96 在 CaP 上的粘附、氧化应激损伤后的活力和增殖行为，该过程从 Velvet Antler 中分离得到 VAP。这种方法可将功能分子的保留率提高到 28 天，并且在没有细胞毒性的情况下支持氧化应激损伤后 RSC96 的粘附和增殖。共沉淀的简单且可重复的方法表明 CaP 是将生物分子功能化用于周围神经修复相关应用的理想载体。
