State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, China; School of Public Health, Guangxi Medical University, Nanning 530021, China.
State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, China.
Toxicol Lett. 2020 Feb 1;319:119-128. doi: 10.1016/j.toxlet.2019.10.009. Epub 2019 Nov 2.
Long-term exposure to fine particulate matter (PM) may cause or exacerbate many diseases, including respiratory inflammation. However, the full mechanism is not yet fully understood. The newly discovered long chain non-coding RNA, though unable to encode proteins, regulates multiple life activities and participates in the development of inflammation. In this study, we set up a cell inflammation model by using normal bronchial 16HBE cells exposed to PM. High-throughput sequencing, as well as real-time fluorescent quantitative PCR detection and validation, was performed on the inflamed cells to evaluate the expression level of long chain noncoding RNA that helped us to identify the LncRNA LOC101927514. Inhibiting LncRNA LOC101927514 expression by RNAi, reflected in a reduction in inflammation, is driven by PM. In addition, we identify LncRNA LOC101927514 to be located within the nucleus and binds to STAT3, altering the inflammatory state of the cells and IL6 and IL8 release. This study identifies that LncRNA LOC101927514 is a new potential target for future treatment of the inflammatory response activated by PM in the respiratory system.
长期暴露于细颗粒物（PM）可能导致或加重许多疾病，包括呼吸道炎症。然而，其完整的机制尚未完全阐明。新发现的长链非编码 RNA 虽然不能编码蛋白质，但可以调节多种生命活动并参与炎症的发生。在这项研究中，我们通过用 PM 暴露正常支气管 16HBE 细胞建立了细胞炎症模型。对炎症细胞进行高通量测序以及实时荧光定量 PCR 检测和验证，以评估有助于我们识别 LncRNA LOC101927514 的长链非编码 RNA 的表达水平。通过 RNAi 抑制 LncRNA LOC101927514 的表达，反映出 PM 驱动的炎症减轻。此外，我们发现 LncRNA LOC101927514 位于核内并与 STAT3 结合，改变细胞的炎症状态并释放 IL6 和 IL8。这项研究表明，LncRNA LOC101927514 是呼吸系统中 PM 激活的炎症反应的未来治疗的一个新的潜在靶点。
