College of Oceanography, Hohai University, 1 Xikang Road, Nanjing, Jiangsu, 210098, China; Postdoctoral Innovation Practice Base, Jiangsu Shuixian Industrial Company Limited, 40 Tonghu Road, Baoying, Yangzhou, Jiangsu, 225800, China.
Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, 250014, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu, 222005, China; College of Marine Science and Engineering, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu, 210023, China.
Fish Shellfish Immunol. 2019 Dec;95:519-527. doi: 10.1016/j.fsi.2019.10.070. Epub 2019 Nov 2.
Kruppel-like factors (KLFs) belong to a family of zinc finger-containing transcription factors that are widely present in eukaryotes. In the present study, a novel KLF from the giant river prawn Macrobrachium rosenbergii (designated as MrKLF) was successfully cloned and characterized. The full-length cDNA of MrKLF was 1799 bp with an open reading frame of 1332 bp that encodes a putative protein of 444 amino acids, including three conserved ZnF_C2H2 domains at the C-terminus. Multiple alignment analysis showed that MrKLF and other crustacean KLFs shared high similarity. Quantitative real-time PCR analysis revealed that MrKLF mRNA was found in different tissues of prawns and detected in the gills, hepatopancreas, and intestines. After the challenge with Vibrio parahaemolyticus and Aeromonas hydrophila, different expression patterns of MrKLF in the gills, intestines, and hepatopancreas were observed. RNA interference analysis indicated that MrKLF was involved in regulating the expression of four antimicrobial peptides, namely, Crustin (Crus) 2, Crus8, anti-lipopolysaccharide factor (ALF) 1, and ALF3. These results help promote research on M. rosenbergii innate immunity.
Kruppel 样因子（KLFs）属于一类含锌指的转录因子家族，广泛存在于真核生物中。本研究成功克隆并鉴定了罗氏沼虾（Macrobrachium rosenbergii）中的一种新型 KLF（命名为 MrKLF）。MrKLF 的全长 cDNA 为 1799bp，开放阅读框为 1332bp，编码一个由 444 个氨基酸组成的假定蛋白，包括 C 末端的三个保守 ZnF_C2H2 结构域。多重比对分析表明，MrKLF 与其他甲壳类动物的 KLF 具有高度相似性。定量实时 PCR 分析显示，MrKLF mRNA 存在于虾的不同组织中，在鳃、肝胰腺和肠道中均有检测到。在受到副溶血弧菌和嗜水气单胞菌的挑战后，观察到 MrKLF 在鳃、肠和肝胰腺中的表达模式存在差异。RNA 干扰分析表明，MrKLF 参与了四种抗菌肽的表达调控，即 Crus 2、Crus8、抗脂多糖因子（ALF）1 和 ALF3。这些结果有助于促进罗氏沼虾先天免疫的研究。
