Wehling M, Kuhnle U, Witzgall H, Weber P C, Armanini D
Medizinische Klinik Innenstadt, University of Munich, FRG.
Clin Endocrinol (Oxf). 1988 Jan;28(1):67-74. doi: 10.1111/j.1365-2265.1988.tb01205.x.
In three patients with pseudohypoaldosteronism the effects of aldosterone on intracellular sodium and potassium were studied and compared with normal controls in whom aldosterone prevents the loss of sodium and potassium in vitro. Mononuclear leukocytes were incubated with or without aldosterone (1.4 nM) in RPMI-1640 for 1 h at 37 degrees C. After two washes in isotonic MgCl2 the wet cell pellets were weighted and intracellular sodium and potassium determined by flame photometry, results are expressed as mmol/kg wet cells. In the patients intracellular sodium fell from 18, 23 and 29 mmol/kg to 14, 18 and 11 mmol/kg, respectively, in the absence of aldosterone. With aldosterone added to the incubation medium sodium was not different from values obtained without aldosterone (15, 20 and 13 mmol/kg). Corresponding values for potassium were 89, 48 and 75 mmol/kg before and 68, 32 and 51 mmol/kg after incubation without and 69, 36 and 54 mmol/kg after incubation with aldosterone. Thus, incubation with aldosterone did not show an effect on intracellular sodium and potassium as seen in normals. Baseline values of sodium and potassium before the incubation were within the normal range. From these results it is concluded that in patients with pseudohypoaldosteronism the absent or decreased number of mineralocorticoid receptors in mononuclear leucocytes are accompanied by a lack of response of intracellular sodium and potassium to aldosterone in vitro. However, normal baseline intracellular electrolyte concentrations in these patients may indicate that mineralocorticoids are not involved in the maintenance of normal levels of intracellular sodium and potassium.
在三名假性醛固酮减少症患者中，研究了醛固酮对细胞内钠和钾的影响，并与正常对照进行比较，在正常对照中，醛固酮在体外可防止钠和钾的丢失。将单核白细胞在含有或不含有醛固酮（1.4 nM）的RPMI-1640中于37℃孵育1小时。在等渗MgCl2中洗涤两次后，称取湿细胞沉淀的重量，并通过火焰光度法测定细胞内钠和钾，结果以mmol/kg湿细胞表示。在无醛固酮的情况下，患者细胞内钠分别从18、23和29 mmol/kg降至14、18和11 mmol/kg。在孵育培养基中添加醛固酮后，钠含量与未添加醛固酮时的值无差异（15、20和13 mmol/kg）。孵育前和孵育后无醛固酮时钾的相应值分别为89、48和75 mmol/kg以及68、32和51 mmol/kg，孵育有醛固酮时为69、36和54 mmol/kg。因此，与正常情况不同，用醛固酮孵育未显示对细胞内钠和钾有影响。孵育前钠和钾的基线值在正常范围内。从这些结果可以得出结论，在假性醛固酮减少症患者中，单核白细胞中盐皮质激素受体数量的缺乏或减少伴随着体外细胞内钠和钾对醛固酮缺乏反应。然而，这些患者正常的细胞内电解质基线浓度可能表明盐皮质激素不参与维持细胞内钠和钾的正常水平。
