Lee Youngsun, Choi Hye Young, Kwon Ara, Park Hyeyeon, Park Mi-Hyun, Kim Ji-Won, Kim Min Jung, Kim Yong-Ou, Kwak Sungwook, Koo Soo Kyung
Division of Intractable Diseases, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea.
Department of Endocrinology & Metabolism, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Stem Cell Res. 2019 Dec;41:101632. doi: 10.1016/j.scr.2019.101632. Epub 2019 Oct 16.
PDX1 plays a crucial role in the development and maintenance of β-cells and directly regulates pancreatic β-cell-specific transcription factors by binding to the insulin gene. Here, we introduced an EGFP reporter into the C-terminus of PDX1 in KSCBi005-A human induced pluripotent stem cells through homologous recombination using CRISPR/Cas9 nuclease. The cells had a normal karyotype, expressed several pluripotency markers, and maintained their differentiation potential. KSCBi005-A-3 cells can be used to monitor PDX1 expression in live cells during β-cell differentiation; the cell line has been registered at the National Stem Cell Bank, Korea National Institute of Health.
PDX1在β细胞的发育和维持中起关键作用，并通过与胰岛素基因结合直接调节胰腺β细胞特异性转录因子。在此，我们使用CRISPR/Cas9核酸酶通过同源重组将EGFP报告基因导入KSCBi005-A人诱导多能干细胞中PDX1的C末端。这些细胞具有正常的核型，表达几种多能性标志物，并保持其分化潜能。KSCBi005-A-3细胞可用于监测β细胞分化过程中活细胞中PDX1的表达；该细胞系已在韩国国立卫生研究院国家干细胞库注册。
