Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
College of Science, Liaoning Technical University, Fuxin 123000, China.
Toxins (Basel). 2019 Nov 1;11(11):636. doi: 10.3390/toxins11110636.
, as a natural bio-preservative, has attracted a great deal of attention in recent years. In this study, 22 L strains were tested against the aflatoxin-producing fungus, ; strain IAMU80070 showed the highest antifungal activity. At a concentration of 5 × 10 colony-forming units (CFU) mL, it completely inhibited growth and decreased aflatoxin production by 93%. Furthermore, ultrastructural examination showed that IAMU80070 destroyed the cellular structure of hyphae and spores. To explore the inhibitory effect of IAMU80070 on at the transcriptional level, transcriptome data were obtained and subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The aflatoxin biosynthetic process was the most significantly downregulated functional category, while genes implicated in the synthesis and organization of cell wall polysaccharides were upregulated. Quantitative real-time PCR results verified the credibility and reliability of the RNA sequencing data. These results provided insight into the transcriptome of in response to the antagonistic effects of IAMU80070.
作为一种天然生物防腐剂，近年来受到了极大的关注。在这项研究中，对 22 株 L 菌株进行了抗产黄曲霉真菌的测试；菌株 IAMU80070 表现出最高的抗真菌活性。在 5×10 个菌落形成单位 (CFU) mL 的浓度下，它完全抑制了 的生长，并将黄曲霉毒素的产量降低了 93%。此外，超微结构检查显示 IAMU80070 破坏了菌丝和孢子的细胞结构。为了探索 IAMU80070 在转录水平上对 的抑制作用，获得了转录组数据，并进行了基因本体论 (GO) 和京都基因与基因组百科全书 (KEGG) 分析。黄曲霉毒素生物合成过程是下调最显著的功能类别，而参与细胞壁多糖合成和组织的基因则上调。实时定量 PCR 结果验证了 RNA 测序数据的可信度和可靠性。这些结果为 对 IAMU80070 拮抗作用的转录组提供了深入了解。
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