Hussein J M, Myovela H, Tibuhwa D D
Department of Molecular Biology and Biotechnology, University of Dar es Salaam, P.O. Box 35179, Dar es Salaam, Tanzania.
Saudi J Biol Sci. 2024 Apr;31(4):103958. doi: 10.1016/j.sjbs.2024.103958. Epub 2024 Feb 15.
Endophytic fungi that inhabit medicinal plants are microbial resources renowned for having compounds analogous to those produced by their host plants. This study aimed to describe the diversity of endophytic fungi found in Kunth. To better understand the diversity of foliar endophytic fungi found in the leaves of the medicinal plant , we isolated and characterized endophytic by using both morphological and molecular methods employing ITS markers. The antimicrobial activity of endophytic fungi against common human pathogens and was also investigated. A Total of 16 endophytic fungi were successfully isolated from leaves and classified into five orders of Pezizomycotina based on the phylogenic analyses; (56%) (19%) (6%) (13%) and (6%). The antimicrobial activity of crude extracts from fungal endophyte against , and revealed that three isolates; were the most potent, while and displayed no growth inhibition property against the tested organism. The diversity indices were calculated by using the Shannon-Wiener, Margalef, and Simpson indices. The diversity indices analysis revealed an abundance of species diversity, where the dominant species were , and This study describes the diversity of endophytic fungi found in and emphasizes their potential as a source of novel bioactive compounds. More research on phytochemical composition and antimicrobial activity is ongoing to correlate the traditional uses and scientific findings.
栖息于药用植物中的内生真菌是一种微生物资源，因其产生的化合物与宿主植物产生的化合物类似而闻名。本研究旨在描述在Kunth中发现的内生真菌的多样性。为了更好地了解在这种药用植物叶片中发现的叶内生真菌的多样性，我们使用形态学和分子方法（采用ITS标记）对内生真菌进行了分离和鉴定。还研究了内生真菌对常见人类病原体的抗菌活性。从叶片中成功分离出16株内生真菌，并根据系统发育分析将其分为座囊菌纲的五个目；（56%）（19%）（6%）（13%）和（6%）。真菌内生菌粗提物对、和的抗菌活性表明，三个分离株；是最有效的，而和对测试生物体没有生长抑制特性。使用香农-维纳指数、马加莱夫指数和辛普森指数计算多样性指数。多样性指数分析揭示了丰富的物种多样性，其中优势物种是、和。本研究描述了在中发现的内生真菌的多样性，并强调了它们作为新型生物活性化合物来源的潜力。正在对植物化学成分和抗菌活性进行更多研究，以关联传统用途和科学发现。
