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冬季饲用油菜作物的瘤胃体外发酵和原位降解动力学

Rumen In Vitro Fermentation and In Situ Degradation Kinetics of Winter Forage Brassicas Crops.

作者信息

Daza José, Benavides Daniel, Pulido Rubén, Balocchi Oscar, Bertrand Annick, Keim Juan

机构信息

Animal Production Institute, Faculty of Agricultural Sciences, Universidad Austral de Chile, Valdivia PO Box 567, Chile.

Graduate School, Faculty of Agricultural Sciences, Universidad Austral de Chile, Valdivia PO Box 567, Chile.

出版信息

Animals (Basel). 2019 Nov 1;9(11):904. doi: 10.3390/ani9110904.

Abstract

The aim of the present study was to evaluate the nutritional value, the rumen in vitro fermentation, and the in situ degradation of (L.) ssp. (kales) and (L.) ssp. (swedes) for winter use. Five varieties of each brassica were used in three field replicates and were randomized in a complete block nested design. All forage varieties were harvested at 210 days post-sowing to analyze the chemical composition, in vitro gas production, volatile fatty acid (VFA) production and in situ dry matter (DM) and crude protein (CP) degradability. Kales presented higher DM and neutral detergent fiber (NDF) content ( < 0.01), whereas swedes showed higher CP, metabolizable energy (ME), glucose, fructose, total sugars, NFC, and nonstructural carbohydrate (NSC) content ( < 0.01). The kale and swede varieties differed in their CP and sugar concentrations, whereas the kale varieties differed in their DM and raffinose content. The rates of gas production were higher for swedes than for kales ( < 0.01). No differences between the brassica species ( > 0.05) were observed in the total VFA production, whereas kales had a higher proportion of acetate and swedes had higher proportions of butyrate ( < 0.05). Only the swede varieties showed differences in VFA production ( < 0.05). The soluble fraction "a", potential and effective in situ DM degradability were higher in swedes ( < 0.01), but kales presented greater DM and CP degradation rates. Differences were observed between brassica species in the chemical composition, degradation kinetics, and ruminal fermentation products, whereas differences among varieties within species were less frequent but need to be considered.

摘要

本研究的目的是评估用于冬季的甘蓝(L.)亚种羽衣甘蓝和芜菁甘蓝(L.)亚种芜菁的营养价值、瘤胃体外发酵及原位降解情况。每个芸苔属品种选取5个,进行3次田间重复,并按完全区组嵌套设计随机排列。所有饲草品种均在播种后210天收获,以分析其化学成分、体外产气、挥发性脂肪酸(VFA)产生以及原位干物质(DM)和粗蛋白(CP)降解率。羽衣甘蓝的DM和中性洗涤纤维(NDF)含量较高(P<0.01),而芜菁的CP、代谢能(ME)、葡萄糖、果糖、总糖、NFC和非结构性碳水化合物(NSC)含量较高(P<0.01)。羽衣甘蓝和芜菁品种在CP和糖浓度上存在差异,而羽衣甘蓝品种在DM和棉子糖含量上存在差异。芜菁的产气速率高于羽衣甘蓝(P<0.01)。在总VFA产生方面,未观察到芸苔属物种之间存在差异(P>0.05),而羽衣甘蓝的乙酸比例较高,芜菁的丁酸比例较高(P<0.05)。仅芜菁品种在VFA产生方面存在差异(P<0.05)。芜菁的可溶性部分“a”、潜在和有效原位DM降解率较高(P<0.01),但羽衣甘蓝的DM和CP降解率更高。在化学成分、降解动力学和瘤胃发酵产物方面,芸苔属物种之间存在差异,而物种内品种之间的差异较少见,但仍需考虑。

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