纳米复合包装调节食用菌（金针菇）细胞外 ATP 和程序性细胞死亡。
Nanocomposite packaging regulates extracellular ATP and programed cell death in edible mushroom (Flammulina velutipes).
机构信息
College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance & Economics, Nanjing, Jiangsu 210023, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
出版信息
Food Chem. 2020 Mar 30;309:125702. doi: 10.1016/j.foodchem.2019.125702. Epub 2019 Oct 19.
Our previous study indicated that nanocomposite packaging material (Nano-PM) containing nano-Ag, nano-TiO, nano-SiO and nanoattapulgite alleviated postharvest senescence of Flammulina velutipes by regulating respiration and energy metabolism. In this study, extracellular ATP (eATP) and programmed cell death (PCD) were employed as critical factors to further investigate the senescence mechanism of postharvest F. velutipes. Results demonstrated that Nano-PM delayed apyrase activity decrease and stimulated critical oxidative phosphorylation-related gene expression to inhibit eATP content increase, which is a crucial signaling molecule related to delaying senescence. The regulation of eATP resulted in alleviating PCD including chromosomal concentration, DNA fragmentation, Ca influx, high caspase-1 activity and cytochrome c content and leading to high cell viability. Overall, Nano-PM alleviated PCD and postharvest senescence of F. velutipes by regulating extracellular ATP.
我们之前的研究表明，含有纳米银、纳米二氧化钛、纳米二氧化硅和纳米凹凸棒土的纳米复合包装材料（Nano-PM）通过调节呼吸和能量代谢来延缓金针菇的采后衰老。在本研究中，细胞外三磷酸腺苷（eATP）和程序性细胞死亡（PCD）被用作关键因素，以进一步研究金针菇采后的衰老机制。结果表明，Nano-PM 延迟了脱氨酶活性的降低，并刺激了关键的氧化磷酸化相关基因的表达，从而抑制了 eATP 含量的增加，eATP 是一种与延缓衰老相关的重要信号分子。eATP 的调节导致了包括染色体浓缩、DNA 片段化、Ca2+内流、高半胱氨酸蛋白酶-1 活性和细胞色素 c 含量在内的 PCD 的缓解，从而导致高细胞活力。总的来说，Nano-PM 通过调节细胞外 ATP 来缓解金针菇的 PCD 和采后衰老。
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