Yu Wancong, Li Shihao, Zheng Bowen, Wang Yuqi, Yu Yue, Wang Yumeng, Zheng Xu, Liu Jiping, Zhang Zhijun, Xue Zhaohui
Biotechnology Research Institute, Tianjin Academy of Agricultural Sciences, 300384 Tianjin, China.
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Food Chem (Oxf). 2022 Jul 6;5:100117. doi: 10.1016/j.fochms.2022.100117. eCollection 2022 Dec 30.
Transcriptome analysis is important for the quality improvement of edible fungi, however, the effect of polyethylene (PE) packaging on the preservation of at the transcriptome level still needs to be further investigated. In order to elucidate the effect of PE on delaying lignification of , this study focused on exploring effects of PE on enzymes and genes involved in lignification. The results showed that PE packaging delayed the deterioration of phenotype, color difference and weight loss rate of , inhibited lignin and HO content and maintained firmness and cellulose content. The activities of PAL, POD, 4-CL were inhibited, and more laccase expression was activated. Fifty-five differentially expressed genes associated with laccase, multifunctional peroxidase (VP), POD and 4-CL were screened from 10 d, 20 d and 30 d transcriptome data. These results show that PE could inhibit lignification of by up-regulating laccase and VP related genes involved in lignin decomposition and down-regulating the expression of genes involved in lignin synthesis. Meanwhile, we employed Confocal Raman microspectroscopy (CRM) to realize lignin cell level visualization and PE could reduce lignin deposition and weaken the lignin signal bands formed. Therefore, PE can alleviate the lignification of during storage by regulating the expression of specific genes, advancing the understanding of lignification in postharvest at the molecular level, and CRM has the potential to detect the changes of cell wall.
转录组分析对于食用菌品质改良很重要，然而，聚乙烯（PE）包装在转录组水平上对[具体食用菌名称未给出]保鲜的影响仍需进一步研究。为了阐明PE对延缓[具体食用菌名称未给出]木质化的影响，本研究着重探索PE对参与木质化的酶和基因的作用。结果表明，PE包装延缓了[具体食用菌名称未给出]的表型劣变、色差和失重率，抑制了木质素和HO含量，并维持了硬度和纤维素含量。苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）、4-香豆酸辅酶A连接酶（4-CL）的活性受到抑制，同时激活了更多漆酶的表达。从第10天、第20天和第30天的转录组数据中筛选出55个与漆酶、多功能过氧化物酶（VP）、POD和4-CL相关的差异表达基因。这些结果表明，PE可通过上调参与木质素分解的漆酶和VP相关基因以及下调参与木质素合成的基因表达来抑制[具体食用菌名称未给出]的木质化。同时，我们采用共聚焦拉曼光谱（CRM）实现木质素细胞水平可视化，PE可减少木质素沉积并减弱形成的木质素信号带。因此，PE可通过调节特定基因的表达来缓解[具体食用菌名称未给出]贮藏期间的木质化，增进对采后[具体食用菌名称未给出]木质化在分子水平上的理解，并且CRM有潜力检测[具体食用菌名称未给出]细胞壁的变化。
