Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu 41062, Republic of Korea.
Mediators Inflamm. 2019 Oct 10;2019:5914396. doi: 10.1155/2019/5914396. eCollection 2019.
This study is aimed at determining whether Sesamum indicum Linn. beneficially influences FcRI-mediated allergic reactions in RBL-2H3 mast cells; it is also aimed at further investigating Lyn/Fyn and Syk signaling pathways. To examine the antiallergic effect of Linn. extract (SIE), we treated antigen/immunoglobulin E- (IgE-) sensitized mast cells with extracts of various concentrations. We examined the degranulation release and concentrations of inflammatory mediators. Additionally, the expressions of genes involved in the FcRI and arachidonate signaling pathways were examined. SIE inhibited the degranulation and secretion of inflammatory mediators in antigen/IgE-sensitized mast cells. SIE reduced the expressions of FcRI signaling-related genes, such as Syk, Lyn, and Fyn, and the phosphorylation of extracellular signal-regulated kinase in antigen/IgE-sensitized mast cells. Additionally, in late allergic responses, SIE reduced PGD release and COX-2 and cPLA2 phosphorylation expression in FcRI-mediated mast cell activation. Lastly, 250-500 mg/kg SIE significantly attenuated the Ag/IgE-induced passive cutaneous anaphylaxis (PCA) reaction in mice. The potent effect of SIE on RBL-2H3 mast cell activation indicates that the extract could potentially be used as a novel inhibitor against allergic reactions.
本研究旨在确定黑芝麻是否能有益地影响 RBL-2H3 肥大细胞中的 FcRI 介导的过敏反应；还旨在进一步研究 Lyn/Fyn 和 Syk 信号通路。为了研究 Linn. 提取物 (SIE) 的抗过敏作用，我们用不同浓度的提取物处理抗原/免疫球蛋白 E- (IgE-) 致敏的肥大细胞。我们检测脱颗粒释放和炎症介质的浓度。此外，还检测了参与 FcRI 和花生四烯酸信号通路的基因的表达。SIE 抑制了抗原/IgE 致敏的肥大细胞的脱颗粒和炎症介质的分泌。SIE 降低了 FcRI 信号相关基因（如 Syk、Lyn 和 Fyn）的表达，以及抗原/IgE 致敏的肥大细胞中细胞外信号调节激酶的磷酸化。此外，在晚期过敏反应中，SIE 降低了 FcRI 介导的肥大细胞活化中 PGD 释放和 COX-2 和 cPLA2 磷酸化表达。最后，250-500mg/kg 的 SIE 显著减轻了小鼠 Ag/IgE 诱导的被动皮肤过敏反应 (PCA)。SIE 对 RBL-2H3 肥大细胞活化的有效作用表明，该提取物可能可用作新型抗过敏反应抑制剂。
