使用纳米结构荧光化学感应组件对三磷酸腺苷 (ATP) 进行纳摩尔检测。
Nanomolar detection of adenosine triphosphate (ATP) using a nanostructured fluorescent chemosensing ensemble.
机构信息
Department of Chemistry, Nanchang University, Nanchang 330031, P. R. China.
Institute for Advanced Study, Nanchang University, Nanchang 330031, China.
出版信息
Chem Commun (Camb). 2019 Dec 7;55(94):14135-14138. doi: 10.1039/c9cc08054f. Epub 2019 Nov 5.
We report a novel nanostructured chemosensing ensemble PyNp-C13/UD, obtained by self-assembling uranine dye (UD) and an amphiphilic pyridinium salt PyNp-C13. The ensemble was developed for the fluorescence turn-on sensing of ATP in aqueous solutions and inside living cells. The assembly operates via an indicator displacement assay (IDA) method with an ultra-low detection limit of 6.8 nM.
我们报告了一种新型的纳米结构化学感应组合 PyNp-C13/UD，它是通过自组装尿嘧啶染料（UD）和两亲性吡啶鎓盐 PyNp-C13 获得的。该组合是为在水溶液和活细胞中对 ATP 的荧光开启传感而开发的。该组装通过指示剂置换分析（IDA）方法进行操作，检测限低至 6.8 nM。
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