State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China.
Biosensors (Basel). 2024 Oct 8;14(10):488. doi: 10.3390/bios14100488.
In this work, we report a study of a zinc sulfide (ZnS) nanocrystal and reduced graphene oxide (RGO) nanocomposite-based non-enzymatic uric acid biosensor. ZnS nanocrystals with different morphologies were synthesized through a hydrothermal method, and both pure nanocrystals and related ZnS/RGO were characterized with SEM, XRD and an absorption spectrum and resistance test. It was found that compared to ZnS nanoparticles, the ZnS nanoflakes had stronger UV light absorption ability at the wavelength of 280 nm of UV light. The RGO significantly enhanced the electron transfer efficiency of the ZnS nanoflakes, which further led to a better photoelectrochemical property of the ZnS/RGO nanocomposites. The ZnS nanoflake/RGO nanocomposite-based biosensor showed an excellent uric acid detecting sensitivity of 534.5 μA·cm·mM in the linear range of 0.01 to 2 mM and a detection limit of 0.048 μM. These results will help to improve non-enzymatic biosensor properties for the rapid and accurate clinical detection of uric acid.
在这项工作中，我们报告了一种基于硫化锌（ZnS）纳米晶体和还原氧化石墨烯（RGO）纳米复合材料的非酶尿酸生物传感器的研究。通过水热法合成了具有不同形态的 ZnS 纳米晶体，并用 SEM、XRD 和吸收光谱及电阻测试对纯纳米晶体和相关的 ZnS/RGO 进行了表征。结果表明，与 ZnS 纳米颗粒相比，ZnS 纳米薄片在 280nm 紫外光的波长下具有更强的紫外光吸收能力。RGO 显著提高了 ZnS 纳米薄片的电子转移效率，从而进一步提高了 ZnS/RGO 纳米复合材料的光电化学性能。基于 ZnS 纳米薄片/RGO 纳米复合材料的生物传感器在 0.01 至 2mM 的线性范围内表现出优异的尿酸检测灵敏度，为 534.5μA·cm·mM，检测限为 0.048μM。这些结果将有助于改善非酶生物传感器的性能，实现尿酸的快速、准确的临床检测。
