Basara N, Biljanović-Paunović L, Pavlović-Kentera V
Institute for Medical Research, Beograd, Yugoslavia.
Exp Hematol. 1988 Oct;16(9):790-3.
A method for growing erythroid bursts from rat bone marrow cells is presented. The methylcellulose culture technique with rat spleen-conditioned medium was used. Successful growth of rat BFU-E was obtained with 20% fetal bovine serum when anemic rat plasma was used as the source of erythropoietin. Plating efficiency was 60 BFU-E per 2 x 10(5) nucleated cells seeded. The proliferative activity determined by cytosine arabinoside (Ara-C) suicide was about 22%. The effect of chronic hypertransfusion on erythroid colony formation is presented.
本文介绍了一种从大鼠骨髓细胞中培养红系爆式集落的方法。采用了含大鼠脾脏条件培养基的甲基纤维素培养技术。当使用贫血大鼠血浆作为促红细胞生成素来源时，添加20%胎牛血清可成功培养大鼠BFU-E。每接种2×10(5)个有核细胞的接种效率为60个BFU-E。通过阿糖胞苷（Ara-C）自杀法测定的增殖活性约为22%。本文还介绍了慢性大量输血对红系集落形成的影响。
