Ma Guang-Chao, Wang Tie-Shan, Wang Ji, Ma Zhi-Jie, Pu Shi-Biao
Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, China.
Clinical Laboratory Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Biomed Chromatogr. 2020 Mar;34(3):e4739. doi: 10.1002/bmc.4739. Epub 2020 Jan 13.
Allergic rhinitis (AR) negatively affects the healthy lives of many individuals. Most previous studies on AR focused on the expression of cytokines, with only a few analyzing cytokine expression from a metabolomics viewpoint. Therefore, it is worthwhile to study AR at the metabolic level. Consequently, we aimed to identify differential serum biomarkers by metabolomics. In this study, the orthogonal partial least squares discriminant analysis (OPLS-DA) model was applied to characterize the differences in serum samples collected from patients with AR and healthy volunteers. Ten metabolites (except hexadecanoic acid) were found to be altered significantly (p < .05) in the former group, according to results of principal component analysis and OPLS-DA, indicating that these metabolites could be potential biomarkers. MetaboAnalyst 4.0 and pathway enrichment analysis showed that these changes in metabolites mainly involved three pathways, namely, porphyrin and chlorophyll metabolism, arachidonic acid metabolism, and purine metabolism. Our findings may contribute to a better understanding of the potential pathogenesis mechanisms and provide a metabolic evidence for in-depth studies of AR.
变应性鼻炎（AR）对许多人的健康生活产生负面影响。以往大多数关于AR的研究集中在细胞因子的表达上，只有少数研究从代谢组学角度分析细胞因子表达。因此，从代谢水平研究AR是值得的。因此，我们旨在通过代谢组学鉴定差异血清生物标志物。在本研究中，采用正交偏最小二乘判别分析（OPLS-DA）模型来表征从AR患者和健康志愿者收集的血清样本中的差异。根据主成分分析和OPLS-DA的结果，发现前一组中有10种代谢物（十六烷酸除外）有显著变化（p<0.05），表明这些代谢物可能是潜在的生物标志物。MetaboAnalyst 4.0和通路富集分析表明，这些代谢物的变化主要涉及三条通路，即卟啉和叶绿素代谢、花生四烯酸代谢和嘌呤代谢。我们的发现可能有助于更好地理解潜在的发病机制，并为AR的深入研究提供代谢证据。
