Faculty of Pharmacy, Microbiology Department, Eastern Mediterranean University, Famagusta, Turkey.
Istanbul Faculty of Medicine, Medical Microbiology Department, Istanbul University, Istanbul, Turkey.
APMIS. 2020 Jan;128(1):41-47. doi: 10.1111/apm.13005.
Campylobacteriosis is one of the most frequently reported zoonoses worldwide. The well-documented increase in the ciprofloxacin resistance has increased the importance of rapid detection of the resistance. The incidence of ciprofloxacin resistance was investigated using real-time PCR. Identification of one hundred and fifty-eight strains was performed by PCR. Minimum inhibitory concentration (MIC) of ciprofloxacin was determined by Epsilometer test. Following the confirmation of the efficiencies of singleplex real-time PCR methods using two different probes, a cytosine to thymine point mutation at codon 86 was detected by allelic discrimination. Of the 158 strains, 114 (72.2%) were determined to be resistant to ciprofloxacin. The MIC and the MIC of ciprofloxacin were found to be 8 and ≥32 mg/L, respectively. By real-time PCR, the presence of the mutation was confirmed in all, but one, resistant strains and the absence of the mutation was demonstrated in all, but one, susceptible strains. The rate of resistance is high among C. jejuni strains and ciprofloxacin should not be used in the treatment of such infections in Turkey. A cytosine to thymine mutation is the most frequently detected mechanism for the resistance. Real-time PCR can be used for the quick screening of the resistance.
空肠弯曲菌肠炎是全世界最常报告的动物源性传染病之一。已充分记录的环丙沙星耐药性增加，增加了快速检测耐药性的重要性。使用实时 PCR 研究了环丙沙星耐药性的发生率。通过 PCR 鉴定了 158 株菌。通过 Epsilometer 试验确定了环丙沙星的最小抑菌浓度（MIC）。在使用两种不同探针确认了单重实时 PCR 方法的效率后，通过等位基因鉴别检测到密码子 86 处的胞嘧啶到胸腺嘧啶点突变。在 158 株菌株中，有 114 株（72.2%）被确定对环丙沙星具有耐药性。MIC 和环丙沙星的 MIC 分别为 8 和≥32mg/L。通过实时 PCR，在所有耐药菌株中均证实了该突变的存在，而在所有敏感菌株中均未发现该突变。在土耳其，空肠弯曲菌菌株的耐药率很高，不应将环丙沙星用于此类感染的治疗。胞嘧啶到胸腺嘧啶的突变是最常检测到的耐药机制。实时 PCR 可用于快速筛选耐药性。
