Teksoy Nermin, Ilktac Mehmet, Ongen Betigul
Medical Microbiology Department, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey.
Faculty of Pharmacy, Eastern Mediterranean University, via Mersin 10 Turkey, Famagusta 99628, Cyprus.
Healthcare (Basel). 2023 Sep 16;11(18):2562. doi: 10.3390/healthcare11182562.
is one of the most commonly reported foodborne bacteria worldwide. Although and have been reported to be responsible for the great majority of campylobacteriosis, the burden of infections by species other than and have been increasing as a result of a transition to diagnostic test methods that enable the isolation of emerging species. The aim of the present study was to recover , , and emerging species from the stool samples of 500 patients with gastroenteritis and 100 healthy subjects via the use of a filtration method and culture techniques using Butzler agar and mCCDA under a microaerobic or hydrogen-enriched atmosphere, identify the species by multiplex PCR methods and assess the significance of emerging species in enteric diseases. Thirty-one (6.2%) spp. were isolated from the stool samples of diarrheic patients but none from healthy individuals. Of 31 isolates, 21 (67.8%), nine (29%), and one (3.2%) were identified as , , and by multiplex PCR, respectively. The filtration method was superior to the culture technique using mCCDA under a microaerobic atmosphere. was evaluated as the etiology of gastroenteritis as a result of laboratory and clinical evaluations. The present study was the first to indicate that emerging species are rarely detected and is linked to acute gastroenteritis in Turkey where additional studies are warranted to clarify the significance of emerging species in gastroenteritis.
是全球报告最常见的食源细菌之一。虽然据报道和是大多数弯曲杆菌病的病因，但由于向能够分离新出现物种的诊断测试方法的转变，除和之外的其他物种的感染负担一直在增加。本研究的目的是通过使用过滤方法以及在微需氧或富氢气氛下使用布茨勒琼脂和mCCDA的培养技术，从500例肠胃炎患者和100例健康受试者的粪便样本中分离、和新出现的物种，通过多重PCR方法鉴定这些物种，并评估新出现物种在肠道疾病中的重要性。从腹泻患者的粪便样本中分离出31株（6.2%）弯曲杆菌属菌株，但健康个体中未分离出。在31株分离株中，通过多重PCR分别鉴定出21株（67.8%）、9株（29%）和1株（3.2%）为、和。过滤方法优于在微需氧气氛下使用mCCDA的培养技术。经过实验室和临床评估，被评估为肠胃炎的病因。本研究首次表明，在土耳其很少检测到新出现的弯曲杆菌物种，并且与急性肠胃炎有关，在土耳其有必要进行更多研究以阐明新出现物种在肠胃炎中的重要性。
