Department of Chemistry , University of Toronto , 80 St. George Street , Toronto , ON M5S 3H6 , Canada.
SPARC BioCentre-The Hospital for Sick Children , The Peter Gilgan Centre for Research and Learning , 686 Bay Street, 21st Floor , Toronto , ON M5G 0A4 , Canada.
Bioconjug Chem. 2019 Nov 20;30(11):2805-2810. doi: 10.1021/acs.bioconjchem.9b00559. Epub 2019 Nov 6.
An enzyme-catalyzed reporter deposition stain has been developed for Imaging Mass Cytometry (IMC). The reagent consists of an alkaline phosphatase substrate tethered to a tellurophene which serves as reporter group for mass cytometry. Upon phosphate hydrolysis, a quinone methide is released which covalently labels local nucleophiles. This strategy is a useful complement to heavy isotope antibody conjugates as it facilitates signal amplification for low-abundance biomarker detection. The workflow is conveniently integrated with standard IMC antibody staining to allow multiparametric antigen detection.
现已开发出一种用于成像质谱细胞术（IMC）的酶催化报告物沉积染色剂。该试剂由碱性磷酸酶底物与碲吩连接而成，碲吩作为质谱细胞术的报告基团。磷酸水解后，释放出醌甲，它可使局部亲核试剂发生共价标记。与重同位素抗体缀合物相比，这种策略是一种有用的补充，因为它有利于低丰度生物标志物检测的信号放大。该工作流程与标准 IMC 抗体染色方便地集成在一起，以允许多参数抗原检测。
