Department of Occupational and Environmental Health, Chongqing Medical University, China.
Institute of Life Sciences, Chongqing Medical University, China.
FEBS Lett. 2020 Mar;594(5):823-840. doi: 10.1002/1873-3468.13663. Epub 2019 Nov 26.
Selective autophagy for the elimination of aberrant mitochondria, termed mitophagy, can be regulated by the kinase PINK1 and the ubiquitin ligase Parkin. The lysosome-associated membrane protein 2 (LAMP-2) plays diverse functions in non-selective autophagy, chaperone-mediated autophagy and selective autophagy for the degradation of RNA/DNA. In the present study, we investigated whether LAMP-2 plays important roles during PINK1/Parkin-mediated mitophagy. The results obtained clearly show that knockdown of LAMP-2 does not cause defects in mitophagy in HeLa cells stably expressing Parkin, indicating that LAMP-2 is dispensable for PINK1/Parkin-mediated mitophagy. The present study is the first to determine the potential role of LAMP-2 in PINK1/Parkin-mediated mitophagy, thereby providing more insight into the sophisticated process of mitophagy.
选择性自噬可以消除异常的线粒体，称为线粒体自噬，可以被激酶 PINK1 和泛素连接酶 Parkin 调节。溶酶体相关膜蛋白 2（LAMP-2）在非选择性自噬、伴侣介导的自噬和选择性自噬降解 RNA/DNA 中发挥多种功能。在本研究中，我们研究了 LAMP-2 是否在 PINK1/Parkin 介导的线粒体自噬中发挥重要作用。研究结果清楚地表明，在稳定表达 Parkin 的 HeLa 细胞中敲低 LAMP-2 不会导致线粒体自噬缺陷，表明 LAMP-2对于 PINK1/Parkin 介导的线粒体自噬是可有可无的。本研究首次确定了 LAMP-2 在 PINK1/Parkin 介导的线粒体自噬中的潜在作用，从而更深入地了解了线粒体自噬这一复杂过程。
