Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
Laboratory for Vaccine and Immunotherapeutic, Institute of Biosciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
Int J Nanomedicine. 2019 Aug 19;14:6661-6678. doi: 10.2147/IJN.S214923. eCollection 2019.
BACKGROUND: Cancer treatments are being continually developed. Increasingly more effective and better-targeted treatments are available. As treatment has developed, the outcomes have improved. PURPOSE: In this work, polyethylene glycol (PEG), layered double hydroxide (LDH) and 5-fluorouracil (5-FU) were used as a stabilizing agent, a carrier and an anticancer active agent, respectively. CHARACTERIZATION AND METHODS: Magnetite nanoparticles (Fe3O4) coated with polyethylene glycol (PEG) and co-coated with 5-fluorouracil/Mg/Al- or Zn/Al-layered double hydroxide were synthesized by co-precipitation technique. Structural, magnetic properties, particle shape, particle size and drug loading percentage of the magnetic nanoparticles were investigated by XRD, TGA, FTIR, DLS, FESEM, TEM, VSM, UV-vis spectroscopy and HPLC techniques. RESULTS: XRD, TGA and FTIR studies confirmed the formation of Fe3O4 phase and the presence of iron oxide nanoparticles, polyethylene glycol, LDH and the drug for all the synthesized samples. The size of the nanoparticles co-coated with Mg/Al-LDH is about 27 nm compared to 40 nm when they were co-coated with Zn/Al-LDH, with both showings near uniform spherical shape. The iron oxide nanoparticles retain their superparamagnetic property when they were coated with polyethylene glycol, polyethylene glycol co-coated with Mg/Al-LDH and polyethylene glycol co-coated with Zn/Al-LDH with magnetic saturation value of 56, 40 and 27 emu/g, respectively. The cytotoxicity study reveals that the anticancer nanodelivery system has better anticancer activity than the free drug, 5-FU against liver cancer HepG2 cells and at the same time, it was found to be less toxic to the normal fibroblast 3T3 cells. CONCLUSION: These are unique core-shell nanoparticles synthesized with the presence of multiple functionalities are hoped can be used as a multifunctional nanocarrier with the capability of targeted delivery using an external magnetic field and can also be exploited as hypothermia for cancer cells in addition to the chemotherapy property.
背景：癌症治疗方法在不断发展。越来越多的更有效和更有针对性的治疗方法正在出现。随着治疗方法的发展，治疗效果也得到了改善。
目的：本研究以聚乙二醇（PEG）、层状双氢氧化物（LDH）和 5-氟尿嘧啶（5-FU）分别作为稳定剂、载体和抗癌活性剂，制备了载 5-FU 的 PEG 修饰的 LDH/Fe3O4 磁性纳米复合材料。
表征方法：采用共沉淀法制备了聚乙二醇（PEG）修饰的、共包覆 5-氟尿嘧啶/ Mg/Al-或 Zn/Al-层状双氢氧化物的四氧化三铁（Fe3O4）磁性纳米粒子。采用 XRD、TGA、FTIR、DLS、FESEM、TEM、VSM、UV-vis 光谱和 HPLC 技术对磁性纳米粒子的结构、磁性能、颗粒形状、粒径和载药量进行了研究。
结果：XRD、TGA 和 FTIR 研究证实了 Fe3O4 相的形成和铁氧化物纳米粒子、聚乙二醇、LDH 和药物的存在。共包覆 Mg/Al-LDH 的纳米粒子的粒径约为 27nm，而共包覆 Zn/Al-LDH 的纳米粒子的粒径约为 40nm，两者均呈现出近球形的均匀形状。当氧化铁纳米粒子被聚乙二醇、聚乙二醇共包覆 Mg/Al-LDH 和聚乙二醇共包覆 Zn/Al-LDH 包覆时，仍保持超顺磁性，其饱和磁化强度分别为 56、40 和 27 emu/g。细胞毒性研究表明，与游离药物 5-FU 相比，载药纳米递药系统对肝癌 HepG2 细胞具有更好的抗癌活性，同时对正常成纤维细胞 3T3 细胞的毒性也较低。
结论：这些具有多种功能的独特核壳纳米粒子有望被用作多功能纳米载体，通过外加磁场进行靶向输送，并且还可以利用外加磁场的磁热疗特性用于癌症治疗。
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