Sani Usman Muhammad, Hussein Mohd Zobir, Fakurazi Sharida, Masarudin Mas Jaffri, Ahmad Saad Fathinul Fikri
Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Serdang 43400, Selangor, Malaysia.
Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Serdang 43400, Selangor, Malaysia.
Nanomaterials (Basel). 2017 Aug 31;7(9):244. doi: 10.3390/nano7090244.
We have developed gadolinium-based theranostic nanoparticles for co-delivery of drug and magnetic resonance imaging (MRI) contrast agent using Zn/Al-layered double hydroxide as the nanocarrier platform, a naturally occurring phenolic compound, gallic acid (GA) as therapeutic agent, and Gd(NO₃)₃ as diagnostic agent. Gold nanoparticles (AuNPs) were grown on the system to support the contrast for MRI imaging. The nanoparticles were characterized using techniques such as Hi-TEM, XRD, ICP-ES. Kinetic release study of the GA from the nanoparticles showed about 70% of GA was released over a period of 72 h. The in vitro cell viability test for the nanoparticles showed relatively low toxicity to human cell lines (3T3) and improved toxicity on cancerous cell lines (HepG2). A preliminary contrast property test of the nanoparticles, tested on a 3 Tesla MRI machine at various concentrations of GAGZAu and water (as a reference) indicates that the nanoparticles have a promising dual diagnostic and therapeutic features to further develop a better future for clinical remedy for cancer treatment.
我们以锌/铝层状双氢氧化物作为纳米载体平台、天然存在的酚类化合物没食子酸（GA）作为治疗剂、硝酸钆（Gd(NO₃)₃）作为诊断剂，开发了用于药物和磁共振成像（MRI）造影剂共递送的钆基诊疗纳米颗粒。在该体系上生长金纳米颗粒（AuNPs）以增强MRI成像的对比度。使用高分辨透射电子显微镜（Hi-TEM）、X射线衍射（XRD）、电感耦合等离子体发射光谱（ICP-ES）等技术对纳米颗粒进行了表征。纳米颗粒中GA的动力学释放研究表明，在72小时内约70%的GA被释放。纳米颗粒的体外细胞活力测试表明，其对人细胞系（3T3）毒性相对较低，对癌细胞系（HepG2）毒性有所增强。在3特斯拉MRI机器上以不同浓度的GAGZAu和水（作为对照）对纳米颗粒进行的初步对比度性能测试表明，这些纳米颗粒具有良好的双重诊断和治疗特性，有望为癌症治疗的临床补救措施开拓更美好的未来。
