Suh Seung-Man, Park Saet-Byul, Kim Mi-Ju, Kim Hae-Yeong
Institute of Life Sciences and Resources, Department of Food Science and Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin, 17104 Republic of Korea.
Food Sci Biotechnol. 2019 Mar 16;28(5):1593-1598. doi: 10.1007/s10068-019-00591-y. eCollection 2019 Oct.
Fruit allergies have become more common in recent years, and are now a serious health problem. In this study, a multiplex PCR assay was used to detect potential fruit allergens causing food allergy labeling in Korea. For the detection of these allergens, specific primer pairs were designed to amplify the allergen-coding genes (tomato), (apple), (peach) and (kiwi), and primer pair targeting the 18S ribosomal RNA gene was additionally used as an endogenous control. Primer specificity was assessed with 23 plant species. A mixture of DNA from the four fruits was serially diluted and used to determine the sensitivity of the multiplex PCR assay, which was approximately 0.08 ng. Eleven commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. This assay is expected to be a specific and efficient method for detecting fruit allergens in foods.
近年来，水果过敏变得越来越普遍，如今已成为一个严重的健康问题。在本研究中，采用多重聚合酶链反应（PCR）分析法检测韩国食品过敏标签中潜在的水果过敏原。为检测这些过敏原，设计了特异性引物对以扩增过敏原编码基因（番茄）、（苹果）、（桃子）和（猕猴桃），并另外使用靶向18S核糖体RNA基因的引物对作为内源性对照。用23种植物物种评估引物特异性。将四种水果的DNA混合物进行系列稀释，并用于确定多重PCR分析法的灵敏度，其约为0.08纳克。评估了11种商业水果产品以验证多重PCR分析法的适用性。该分析法有望成为检测食品中水果过敏原的一种特异性且高效的方法。
