Department of Basic Medical Sciences, Clinical College of Anhui Medical University, Hefei 230031, China 2 School of Life Sciences, Anhui Medical University, Hefei 230032, China.
School of Life Sciences, Anhui Medical University, Hefei 230032, China.
Genes (Basel). 2024 Jul 22;15(7):965. doi: 10.3390/genes15070965.
Ovarian cancer (OC) is one of the most commonplace gynecological malignancies. This study explored the effects of resveratrol (RES) on OC cell proliferation and apoptosis. Proliferation activity was measured for A2780 cells treated with RES for 24 h and 48 h at concentrations of 0, 10, 25, 50, 75, 100, 150, 200, and 300 μM. RNA sequencing (RNA-seq) was performed to analyze the circular RNA (circRNA), microRNA (miRNA), and messenger RNA (mRNA) expression spectrum. The differentially expressed genes included 460 circRNAs, 1988 miRNAs, and 1671 mRNAs, and they were subjected to analyses including Gene Ontology, the Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome enrichment. We selected signaling pathways enriched in the cell processes by mRNA KEGG, comprehensively analyzed the circRNA-miRNA-mRNA regulatory network, and verified several miRNAs expressed in the regulatory network diagram using the quantitative real-time polymerase chain reaction. The data showed that the cell proliferation of A2780 cells treated with RES for 24 h or 48 h decreased with increasing concentrations of RES. The circRNA-miRNA-mRNA regulatory network that we constructed provides new insights into the ability of RES to inhibit cell proliferation and promote apoptosis in A2780 cells.
卵巢癌（OC）是最常见的妇科恶性肿瘤之一。本研究探讨了白藜芦醇（RES）对 OC 细胞增殖和凋亡的影响。用 RES 处理 A2780 细胞 24 h 和 48 h，浓度分别为 0、10、25、50、75、100、150、200 和 300 μM，测量细胞增殖活性。进行 RNA 测序（RNA-seq）分析环状 RNA（circRNA）、微小 RNA（miRNA）和信使 RNA（mRNA）表达谱。差异表达基因包括 460 个 circRNA、1988 个 miRNA 和 1671 个 mRNA，并进行了基因本体论、京都基因与基因组百科全书（KEGG）和 Reactome 富集分析。我们选择了细胞过程中富含 mRNA KEGG 的信号通路，综合分析了 circRNA-miRNA-mRNA 调控网络，并使用定量实时聚合酶链反应验证了调控网络图中表达的几种 miRNA。结果表明，RES 处理 A2780 细胞 24 h 或 48 h 后，细胞增殖随 RES 浓度的增加而降低。我们构建的 circRNA-miRNA-mRNA 调控网络为 RES 抑制 A2780 细胞增殖和促进凋亡的能力提供了新的见解。
