Van Oijen Guido W, Croughs Peter D, Hagenaars Tjebbe, Verhofstad Michael H J, Van Lieshout Esther M M
Trauma Research Unit Department of Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Department of Microbiology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
J Bone Jt Infect. 2019 Sep 18;4(5):216-222. doi: 10.7150/jbji.35629. eCollection 2019.
Since 2009, the IlluminOss System is being used as an intramedullary fracture treatment. The system is characterized by the use of blue light to polymerize liquid monomer after its infusion in a polyethylene terephthalate balloon. Very few infections of the material have been observed, which might be explained by the possible antimicrobial side-effect of the blue light used in this intramedullary fracture stabilization system. This study aimed to assess this antimicrobial (side-)effect on . A suspension of 1.5 x 10 CFU/ml of 8325-4 was placed into five, custom made, black delrin cylinders. The implant was placed into the cylinders and the light source was activated for 200, 400, 600, 800, or 1,000 seconds. 100 µL of the light exposed suspension was grafted on blood agar and placed in a 35 degrees Celsius incubator for 24 hours. Colonies on each agar plate were counted and compared to the control plates (no blue light exposure). The control plates showed a mean of 85 ± 15 colonies per plate. A statistically significant decrease was observed after 600 seconds of exposure time; mean colony count of 63 ± 4 (p <0.05). The absolute reduction was 24 ± 14 after 600 seconds exposure time. At 800 and 1,000 seconds, no statistically significant reduction was found compared with the control plates (means 72 ± 10 and 83 ± 14 colonies, respectively). In this study only a temporary reduction of was observed. If future research regarding the antimicrobial characteristics of blue light used in the IlluminOss System is desired, it should focus on the need for oxygen and its availability and the dose and manner of applying the light.
自2009年以来，IlluminOss系统一直被用作髓内骨折治疗手段。该系统的特点是在聚对苯二甲酸乙二酯球囊中注入液体单体后，利用蓝光使其聚合。观察到该材料引起的感染极少，这可能是由于这种髓内骨折固定系统中使用的蓝光具有潜在的抗菌副作用。本研究旨在评估这种抗菌（副）作用。将浓度为1.5×10 CFU/ml的8325 - 4菌悬液放入五个定制的黑色聚甲醛圆柱体中。将植入物放入圆柱体中，光源激活200、400、600、800或1000秒。取100µL经光照处理的菌悬液接种于血琼脂平板上，置于35摄氏度培养箱中培养24小时。对每个琼脂平板上的菌落进行计数，并与对照平板（未暴露于蓝光）进行比较。对照平板平均每平板有85±15个菌落。暴露600秒后观察到有统计学意义的减少；平均菌落数为63±4（p<0.05）。暴露600秒后的绝对减少量为24±14。在800秒和1000秒时，与对照平板相比未发现有统计学意义的减少（平均菌落数分别为72±10和83±14）。在本研究中仅观察到了的暂时减少。如果需要对IlluminOss系统中使用的蓝光的抗菌特性进行未来研究，应关注氧气的需求及其可用性以及光照的剂量和应用方式。
