Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, 200011, China.
Division of Orthodontics, College of Dentistry, The Ohio State University, Rm 4088 Postle Hall, 305 W 12th Ave, Columbus, OH, 43210, USA.
Aesthetic Plast Surg. 2020 Feb;44(1):191-200. doi: 10.1007/s00266-019-01494-3. Epub 2019 Sep 25.
The size and shape of the chin strongly influence facial profile and harmony. The current correction of chin deficiency mostly relies on genioplasty surgery involving osteotomy. To avoid osteotomy, one possible alternative is to enhance bone growth at the mental protuberance area with cell sheet transplantation. This study was undertaken to evaluate the efficacy of this approach in a pig model.
Five 4-month-old pigs were included for mandibular bone marrow aspiration and MSC isolation. Triple-layer MSC sheets were then fabricated and utilized using culture-expanded MSCs. Four weeks after bone marrow aspiration, subperiosteal pockets were created on the labial symphyseal surface, followed by transplantation of autogenous MSC sheets to one randomly chosen side with the other side (control) receiving no transplantation. Six weeks after the surgery, the pigs were euthanized and the specimens from both sides were collected for computed tomography (CT) and histological and immunohistochemical analysis. Measurements between the experimental and control sides were compared using paired t tests.
MSC sheet fabrication and transplantation were reliably conducted. The labial cortical bone thickness increased significantly with MSC sheet transplantation by an average of 2 mm (p = 0.0001). The average measurements of mineral apposition rate and cell proliferation at the cell sheet side tended to be higher than the control side although the differences did not reach statistical significance (p = 0.1-0.2). Tissue mineral density measurements from CT images and bone volume fraction (BV/TV) measurements from histologic images were identical between the two sides (p > 0.5).
These data provide a proof of concept that autologous MSC sheets may be transplanted to the subperiosteal region of the mandibular symphysis to stimulate local surface bone growth.
This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
下巴的大小和形状强烈影响面部轮廓和协调性。目前，下巴缺陷的矫正主要依赖于涉及截骨术的颏成形术。为了避免截骨术，一种可能的替代方法是通过细胞片移植来增强颏突区域的骨生长。本研究旨在评估这种方法在猪模型中的疗效。
纳入 5 头 4 月龄猪进行下颌骨髓抽吸和 MSC 分离。然后制备并使用培养扩增的 MSC 构建三层 MSC 片。骨髓抽吸后 4 周，在唇联合表面创建骨膜下袋，然后将自体 MSC 片移植到一侧，另一侧（对照）不进行移植。手术后 6 周，处死猪，采集双侧标本进行 CT 和组织学及免疫组织化学分析。使用配对 t 检验比较实验组和对照组之间的测量值。
MSC 片的制备和移植可靠进行。MSC 片移植后唇侧皮质骨厚度显著增加，平均增加 2mm（p=0.0001）。尽管差异无统计学意义（p=0.1-0.2），但细胞片侧的矿化沉积率和细胞增殖的平均测量值趋于高于对照组。CT 图像的组织矿物质密度测量值和组织学图像的骨体积分数（BV/TV）测量值在两侧相同（p>0.5）。
这些数据提供了一个概念验证，即自体 MSC 片可移植到下颌联合骨膜下区域，以刺激局部表面骨生长。
本杂志要求作者为每个提交的内容分配一个证据级别，这些级别适用于循证医学排名。这排除了综述文章、书评以及涉及基础科学、动物研究、尸体研究和实验研究的手稿。有关这些循证医学评级的完整描述，请参考目录或在线作者指南 www.springer.com/00266。
