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一种用于测量静水中血吸虫尾蚴密度的差异过滤技术。

A differential filtration technique for the measurement of schistosome cercarial densities in standing waters.

作者信息

Theron A

出版信息

Bull World Health Organ. 1979;57(6):971-5.

PMID:317025
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2395853/
Abstract

The technique of differential filtration allows the detection of schistosome cercariae, with a recovery greater than 80%, in natural waters with a high concentration of microorganisms. This technique makes use of a new type of monofilament polyamide filter Nytrel-TI, with a pore size of 40 mum, allowing filtration of turbid water without clogging of the filter. A three-level prefiltration column (1000, 500 and 200 mum), which retains organisms larger than cercariae, facilitates reading of the filter. The apparatus used weighs less than 3.5 kg; it requires no power supply and can easily be used in the field.

摘要

差异过滤技术能够在微生物浓度高的天然水体中检测血吸虫尾蚴,回收率超过80%。该技术使用了一种新型单丝聚酰胺滤膜Nytrel-TI,孔径为40微米,可过滤浑浊水体而不会堵塞滤膜。一个三级预过滤柱(1000、500和200微米)可截留比尾蚴大的生物,便于读取滤膜结果。所用设备重量不到3.5千克;无需电源,可轻松在野外使用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce14/2395853/98a916f87dd6/bullwho00436-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce14/2395853/98a916f87dd6/bullwho00436-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce14/2395853/98a916f87dd6/bullwho00436-0113-a.jpg

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引用本文的文献

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Pathogens. 2022 May 10;11(5):565. doi: 10.3390/pathogens11050565.
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Challenges and Opportunities Presented by Current Techniques for Detecting Schistosome Infections in Intermediate Host Snails: A Scoping Review.当前检测中间宿主钉螺中血吸虫感染的技术所面临的挑战和机遇:范围综述。
Int J Environ Res Public Health. 2021 May 19;18(10):5403. doi: 10.3390/ijerph18105403.
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The effectiveness of water treatment processes against schistosome cercariae: A systematic review.

本文引用的文献

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Results of exposing mice to schistosomiasis by immersion in natural water.通过将小鼠浸入天然水中使其感染血吸虫病的结果。
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A method for the direct quantitative recovery of Schistosoma mansoni cercariae from natural waters of Puerto Rico.一种从波多黎各天然水域中直接定量回收曼氏血吸虫尾蚴的方法。
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Relation of water velocity to Schistosoma mansoni infection in mice.小鼠体内水流速度与曼氏血吸虫感染的关系
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A continuous flow centrifuge for testing the presence of Bilharzia cercariae in water.一种用于检测水中血吸虫尾蚴的连续流动离心机。
Cent Afr J Med. 1965 Nov;11(11):338-40.
8
New field device for quantitative recovery of Schistosoma mansoni cercariae.用于定量回收曼氏血吸虫尾蚴的新型现场设备。
Public Health Rep (1896). 1967 Mar;82(3):250-2.
9
Laboratory comparison of four cercaria recovery techniques.四种尾蚴回收技术的实验室比较
Bull World Health Organ. 1973;48(1):35-40.
10
Direct filtration for recovery of Schistosoma mansoni cercariae in the field.用于在野外回收曼氏血吸虫尾蚴的直接过滤法。
Bull World Health Organ. 1973;48(1):27-34.