Prentice M A
Ann Trop Med Parasitol. 1984 Apr;78(2):117-27. doi: 10.1080/00034983.1984.11811785.
Modifications to Theron 's (1979) differential filtration technique are described which improve both sensitivity and reliability. Following pre-filtration to remove larger debris and some plankton, the sample is formalized; constant stirring prevents losses due to the cercarial stickiness normally caused by the fixation process. Once fixed, Schistosoma mansoni cercariae are reliably retained and well displayed on nylon monofilament cloth of 50 micrometers pore size. Such a coarse recovery filter permits very large samples to be processed and results in filters which are easy to read. Field comparison showed the method improved sensitivity by a factor of more than 20 times and laboratory evaluation indicated a recovery rate of over 80%.
文中描述了对塞伦（1979年）的差速过滤技术的改进，这些改进提高了灵敏度和可靠性。在进行预过滤以去除较大的碎片和一些浮游生物后，对样品进行固定；持续搅拌可防止因固定过程通常导致的尾蚴粘性而造成损失。一旦固定，曼氏血吸虫尾蚴就能可靠地保留在孔径为50微米的尼龙单丝布上，并能很好地显示出来。这种粗滤网回收率高，能处理非常大的样本，且得到的滤膜易于读数。现场比较表明，该方法的灵敏度提高了20倍以上，实验室评估表明回收率超过80%。
