School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, China.
Beijing Municipal Solid Waste and Chemical Management Center, Beijing, China.
Biotechnol Appl Biochem. 2020 May;67(3):307-316. doi: 10.1002/bab.1848. Epub 2019 Nov 27.
In this study, a halotolerant strain was isolated from high salinity leachate and identified as Bacillus cereus NT-3. It can produce a high concentration of polyhydroxyalkanoates (PHAs) with no significant changes when NaCl concentration is up to 50 g/L. FTIR and NMR spectra of PHAs synthesized by Bacillus cereus NT-3 were similar to the standard or previous results. Effluent from acidogenic fermentation of food waste and pure volatile fatty acids (VFAs) mixture was used as carbon source to check the effect of non-VFAs compounds of the effluent on PHAs production. The maximum PHAs production was 0.42 g/L for effluent fermentation, whereas it was 0.34 g/L for pure VFAs fermentation, indicating that bacteria could use actual effluent in a better way. Furthermore, a mathematical model was established for describing kinetic behavior of bacteria using different carbon sources. These results provided a promising approach for PHAs biosynthesis with a low-cost carbon source.
在这项研究中，从高盐度渗滤液中分离出一株耐盐菌株，并鉴定为蜡状芽孢杆菌 NT-3。当 NaCl 浓度高达 50 g/L 时，它仍能产生高浓度的聚羟基烷酸酯 (PHA)，且没有明显变化。由 Bacillus cereus NT-3 合成的 PHA 的 FTIR 和 NMR 谱与标准谱或先前的结果相似。以产酸发酵的食物废物废水和纯挥发性脂肪酸 (VFA) 混合物作为碳源，以检查废水中非 VFA 化合物对 PHA 生产的影响。废水发酵的最大 PHA 产量为 0.42 g/L，而纯 VFA 发酵的最大 PHA 产量为 0.34 g/L，这表明细菌可以更好地利用实际废水。此外，还建立了一个数学模型来描述使用不同碳源时细菌的动力学行为。这些结果为使用低成本碳源进行 PHAs 生物合成提供了一种很有前途的方法。
