Wang Pan, Qiu Yin-Quan, Chen Xi-Teng, Liang Xiao-Fei, Ren Lian-Hai
School of Food and Chemical Engineering, Beijing Technology and Business University , Beijing , China.
Biosci Biotechnol Biochem. 2019 Oct;83(10):1955-1963. doi: 10.1080/09168451.2019.1630252. Epub 2019 Jun 14.
A metabolomics method was established to analyze changes of intracellular metabolites and study the mechanism for enhancing polyhydroxyalkanoates production by halotolerant bacteria, strain HY-3, using acetic acid as carbon source. Maximum poly(3-hydroxybutyrate) (PHB) contents for the medium with 0.5 g/L and 5.0 g/L of acetic acid were 41.0 ± 0.415% and 49.2 ± 1.21%. Principal components analysis revealed clear metabolic differences in different growth stages and different concentrations of carbon source. According to statistical analysis, 3-hydroxybutyrate (3-HB), serine, threonine, malate, and pyruvate were determined as potential biomarkers for PHB production. Moreover, metabolic pathways analysis indicated that high level of 3-HB in death phase was due to the limitation of carbon source. Metabolism of glycine, serine, and threonine was influential pathway for PHB production among amino acid metabolisms. High levels of organic acids from the TCA cycle could stimulate the carbon source flux into PHB biosynthetic pathway.
建立了一种代谢组学方法，以分析细胞内代谢物的变化，并研究以乙酸为碳源的耐盐细菌HY-3菌株提高聚羟基脂肪酸酯产量的机制。含0.5 g/L和5.0 g/L乙酸的培养基中聚（3-羟基丁酸酯）（PHB）的最大含量分别为41.0±0.415%和49.2±1.21%。主成分分析揭示了不同生长阶段和不同碳源浓度下明显的代谢差异。根据统计分析，3-羟基丁酸（3-HB）、丝氨酸、苏氨酸、苹果酸和丙酮酸被确定为PHB产生的潜在生物标志物。此外，代谢途径分析表明，死亡期3-HB水平较高是由于碳源限制。在氨基酸代谢中，甘氨酸、丝氨酸和苏氨酸的代谢是影响PHB产生的途径。三羧酸循环中高水平的有机酸可刺激碳源流进PHB生物合成途径。
