University of Lille , CNRS, Institut Pasteur de Lille, INSERM U1019, UMR CNRS 8204, Centre d'Immunité et d'Infection de Lille , F-59000 Lille , France.
Bioconjug Chem. 2019 Nov 20;30(11):2967-2973. doi: 10.1021/acs.bioconjchem.9b00661. Epub 2019 Nov 8.
One hallmark of protein chemical synthesis is its capacity to access proteins that living systems can hardly produce. This is typically the case for proteins harboring post-translational modifications such as ubiquitin or ubiquitin-like modifiers. Various methods have been developed for accessing polyubiquitin conjugates by semi- or total synthesis. Comparatively, the preparation of small-ubiquitin-like modifier (SUMO) conjugates, and more particularly of polySUMO scaffolds, is much less developed. We describe hereinafter a synthetic strategy for accessing all SUMO-2/3 dimer combinations.
蛋白质化学合成的一个特点是能够获得生物系统难以产生的蛋白质。对于含有翻译后修饰的蛋白质（如泛素或泛素样修饰物），通常就是这种情况。已经开发出各种方法通过半合成或全合成来获得多泛素缀合物。相比之下，制备小分子泛素样修饰物（SUMO）缀合物，更具体地说是多 SUMO 支架，发展得要少得多。我们在下文描述了一种获得所有 SUMO-2/3 二聚体组合的合成策略。
