Peng Weili, Zhang Zeping, Rong Minzhi, Zhang Mingqiu
Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, GD HPPC Lab, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.
Polymers (Basel). 2019 Nov 7;11(11):1832. doi: 10.3390/polym11111832.
A kind of core-shell hybrid nanoparticle comprised of a hollow mesoporous silica nanoparticles (HMS) core and a copolymer shell bearing N-(3,4-dihydroxyphenethyl) methacrylamide (DMA) and N-isopropylacrylamide (NIPAM) as responsive moieties was prepared. Moreover, the factors that could impact the surface morphology and hierarchical porous structure were discussed. In the presence of Fe, catechol-Fe complexes were formed to achieve pH-responsive polymer shell, combining with thermal-sensitiveness of poly(N-isopropylacrylamide). Doxorubicin (DOX) was applied as a model drug and the behaviors of its loading/release behaviors were investigated to prove the idea. The results exhibited a significant drug loading capacity of 8.6% and embed efficiency of 94.6% under 1 mg ml DOX/PBS solution. In fact, the loading capacity of drug can be easily improved to as high as 28.0% by increasing the DOX concentration. The vitro cytotoxicity assay also indicated that the as-prepared nanoparticles have no significant cytotoxicity on RAW 264.7 cells. The in vitro experiment showed that the cumulative release of DOX was obviously dependent on the temperature and pH values. This pH/temperature-sensitive hollow mesoporous silica nanosphere is expected to have potential applications in controlled drug release.
制备了一种核壳杂化纳米粒子，其由中空介孔二氧化硅纳米粒子（HMS）核和带有N-（3,4-二羟基苯乙基）甲基丙烯酰胺（DMA）和N-异丙基丙烯酰胺（NIPAM）作为响应基团的共聚物壳组成。此外，还讨论了可能影响表面形态和分级多孔结构的因素。在铁存在的情况下，形成邻苯二酚-铁配合物以实现pH响应性聚合物壳，并结合聚（N-异丙基丙烯酰胺）的热敏性。以阿霉素（DOX）作为模型药物，研究了其负载/释放行为以验证该想法。结果表明，在1mg/ml DOX/PBS溶液中，药物负载量高达8.6%，包封效率为94.6%。实际上，通过增加DOX浓度，药物负载量可轻松提高至28.0%。体外细胞毒性试验还表明，所制备的纳米粒子对RAW 264.7细胞无明显细胞毒性。体外实验表明，DOX的累积释放明显取决于温度和pH值。这种pH/温度敏感的中空介孔二氧化硅纳米球有望在药物控释方面具有潜在应用。
