Ochi K, Tsurumi Y, Shigematsu N, Iwami M, Umehara K, Okuhara M
Exploratory Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Ibaraki, Japan.
J Antibiot (Tokyo). 1988 Aug;41(8):1106-15. doi: 10.7164/antibiotics.41.1106.
Streptomyces griseoflavus, a bicozamycin-producing wild type strain and its high-producing one derived from it by multiple (greater than 15) mutagenic treatments, were analyzed physiologically and biochemically. The high-producing strain was characterized by: (1) An increased pool size of amino acids including leucine and isoleucine, precursors for bicozamycin synthesis, (2) an earlier and greater accumulation of intracellular ppGpp, (3) a more accentuated decrease in GTP pool size, (4) a higher specific activity of ornithine transcarbamylase which produces citrulline, (5) an increased ability to form aerial mycelium, and (6) an increased resistance to its own antibiotic. We propose that (1), (2) and (4) may be responsible for the high yields of bicozamycin and, possibly, of some other antibiotics produced by Streptomyces sp.
对产双霉素的野生型菌株灰色链霉菌及其经多次（超过15次）诱变处理后得到的高产菌株进行了生理生化分析。高产菌株的特征如下：（1）包括亮氨酸和异亮氨酸在内的氨基酸库容量增加，这些氨基酸是双霉素合成的前体；（2）细胞内ppGpp更早且积累量更大；（3）GTP库容量下降更为明显；（4）产生瓜氨酸的鸟氨酸转氨甲酰酶的比活性更高；（5）形成气生菌丝体的能力增强；（6）对自身抗生素的抗性增强。我们认为，（1）、（2）和（4）可能是双霉素高产以及链霉菌属产生的其他一些抗生素高产的原因。
