Iseki M, Miyoshi T, Konomi T, Imanaka H
J Antibiot (Tokyo). 1980 May;33(5):488-93. doi: 10.7164/antibiotics.33.488.
The biosynthesis of bicyclomycin by Streptomyces sapporonensis was studied using suspensions of washed mycelium. Nicotinamide and Fe2+ were found to be essential cofactors in the biosynthesis. Production of bicyclomycin was enhanced most effectively in the presence of equal moles of L-leucine and L-isoleucine, which in experiments with radioactively labeled compounds were found to be incorporated into bicyclomycin at equivalent rates. These facts strongly suggest that bicyclomycin biosynthesis involves coupling of equal moles of these two amino acids.
利用洗涤过的菌丝体悬浮液研究了酒红链霉菌合成双环霉素的过程。发现烟酰胺和Fe2+是生物合成中必不可少的辅助因子。在等摩尔的L-亮氨酸和L-异亮氨酸存在下，双环霉素的产量得到最有效的提高，在用放射性标记化合物进行的实验中发现它们以相同的速率掺入双环霉素中。这些事实有力地表明，双环霉素的生物合成涉及这两种氨基酸等摩尔的偶联。
