Chemical Constituents from and Their Ability to Ameliorate Steatosis and Promote Proliferation and Anti-Oxidation In Vitro.

This study describes the chemical constituents of and their ability to ameliorate steatosis and promote proliferation and anti-oxidation in vitro. Together, five known lignan glycosides, (7S,8R)-erythro-syringylglycerol-β-O-4'-sinapyl ether 9-O-β-D-glucopyranoside (), (+)-lyoniresinol-9'-O-gluco-side (), (-)-lyoniresinol-9'-O-glucoside (), picraquassioside C (), and icariside E5 (), were isolated from the . Their structures were elucidated by extensive NMR and high-resolution mass spectrometry analysis and compared with reported data. Oil Red O staining results revealed that compounds , , and attenuated lipid accumulation and lipid metabolic disorders in FFAs (oleate/palmitate, 2:1 ratio, 0.3 mM)-exposed HepG2 cells. The Cell Counting Kit 8 (CCK-8) assay results revealed that compounds and can significantly promote human umbilical vein endothelial cell (HUVEC) proliferation; meanwhile, these compounds did not exhibit significant cytotoxicity against HUVECs. In addition, 2',7'-dichlorofluorescein diacetate (DCFH-DA) staining results revealed that high glucose (HG)-induced reactive oxygen species (ROS) production was abolished by compounds , , and . This is the first report of the isolation of lignan skeletons from the genus with the ability to ameliorate steatosis and promote proliferation and anti-oxidation activities.