Laboratório de Cardiologia Molecular e Celular e Centro de Cardiologia Experimental, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Princesa Isabel avenue, 370 Porto Alegre, RS 90620-001, Brazil; Escola Superior de Educação Física, Universidade Luterana do Brasil, Street. Itacolomi, 3600 - São Vicente, Gravataí, RS 94170-240, Brazil.
Laboratório de Cardiologia Molecular e Celular e Centro de Cardiologia Experimental, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Princesa Isabel avenue, 370 Porto Alegre, RS 90620-001, Brazil.
Cytokine. 2020 Feb;126:154912. doi: 10.1016/j.cyto.2019.154912. Epub 2019 Nov 6.
Stem cell therapy is a promising strategy for recovering of injured cardiac tissue after acute myocardial infarction. The effects promoted by preventive physical training, beneficial for regeneration, are not yet understood on stem cell homing. In the present study, we evaluated the effect of preventive physical training on cell homing activation and associated mechanisms after acute myocardial infarction and therapy with adipose-derived stem cells in spontaneously hypertensive rats (SHR). Forty female SHR were allocated in sedentary (S), sedentary SHAM (S-SHAM), sedentary AMI (S-AMI), sedentary with cell therapy (S-ICT), aerobically trained (T), trained SHAM (T-SHAM), trained AMI (T-AMI) and trained with cell therapy (S-ICT) groups. Cell therapy was performed through the infusion of 2 × 10 ADSC/0.05 mL at the moment of AMI. Molecular markers of cell homing (SDF-1/CXCR4), inflammatory response (myeloperoxidase and cardiac expression of iNOS, gp91phox and NFkB), vasoconstrictor agents (Ang II and ET-1) and an angiogenesis inducer (VEGF) were measured. Functional capacity and echocardiographic parameters were also evaluated. Preventive physical training associated with cell therapy was able to reduce left ventricle ejection fraction losses in infarcted animals. Results demonstrated activation of the SDF-1/CXCR4 axis by physical training, besides a reduction in vasoconstrictor and systemic inflammatory responses. Physical training prior to AMI was able to induce a cardioprotective effect and optimize the reparative mechanism of cell therapy in an animal model of hypertension.
干细胞治疗是急性心肌梗死后修复受损心肌组织的一种很有前途的策略。预防体力训练的有益作用，有利于再生，但其对干细胞归巢的影响尚不清楚。在本研究中，我们评估了预防体力训练对自发性高血压大鼠（SHR）急性心肌梗死后细胞归巢激活及其相关机制的影响，并进行了脂肪来源干细胞治疗。将 40 只雌性 SHR 分为安静组（S）、假手术安静组（S-SHAM）、急性心肌梗死安静组（S-AMI）、急性心肌梗死联合细胞治疗安静组（S-ICT）、有氧训练组（T）、假手术有氧训练组（T-SHAM）、急性心肌梗死有氧训练组（T-AMI）和急性心肌梗死联合细胞治疗有氧训练组（T-ICT）。细胞治疗是在 AMI 发生时通过输注 2×10 ADSC/0.05mL 来完成的。测量了细胞归巢的分子标志物（SDF-1/CXCR4）、炎症反应（髓过氧化物酶和心脏中 iNOS、gp91phox 和 NFkB 的表达）、血管收缩剂（Ang II 和 ET-1）和血管生成诱导剂（VEGF）。还评估了功能能力和超声心动图参数。预防体力训练联合细胞治疗可减少梗死动物左心室射血分数的丧失。结果表明，体力训练激活了 SDF-1/CXCR4 轴，同时降低了血管收缩剂和全身炎症反应。AMI 前进行体力训练能够在高血压动物模型中诱导心脏保护作用，并优化细胞治疗的修复机制。
