Institute of Cardiology of Rio Grande do Sul/University Foundation of Cardiology, Porto Alegre, Rio Grande do Sul, Brazil.
Thyroid Division, Endocrinology Service, Hospital de Clínicas de Porto Alegre/Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
PLoS One. 2019 Sep 12;14(9):e0222334. doi: 10.1371/journal.pone.0222334. eCollection 2019.
We assessed the effects of a short-term exercise training on cardiac function, oxidative stress markers, and type 3 iodothyronine deiodinase (D3) activity in cardiac tissue of spontaneously hypertensive rats (SHR) following experimental myocardial infarction (MI).
Twenty-four SHR (aged 3 months) were allocated to 4 groups: sham+sedentary, sham+trained, MI+sedentary and MI+trained. MI was performed by permanent ligation of the coronary artery. Exercise training (treadmill) started 96 hours after MI and lasted for 4 weeks (~60% maximum effort, 4x/week and 40 min/day). Cardiac function (echocardiography), thioredoxin reductase (TRx), total carbonyl levels, among other oxidative stress markers and D3 activity were measured. A Generalized Estimating Equation was used, followed by Bonferroni's test (p<0.05).
MI resulted in an increase in left ventricular mass (p = 0.002) with decreased cardiac output (22.0%, p = 0.047) and decreased ejection fraction (41%, p = 0.008) as well as an increase in the carbonyl levels (p = 0.001) and D3 activity (33%, p<0.001). Exercise training resulted in a decrease in left ventricular mass, restored cardiac output (34%, p = 0.048) and ejection fraction (20%, p = 0.040), increased TRx (85%, p = 0.007) and reduced carbonyl levels (p<0.001) and D3 activity (p<0.001).
Our short-term exercise training helped reverse the effects of MI on cardiac function. These benefits seem to derive from a more efficient antioxidant response and lower D3 activity in cardiac tissue.
我们评估了短期运动训练对自发性高血压大鼠（SHR）实验性心肌梗死（MI）后心脏功能、氧化应激标志物和 3 型甲状腺素脱碘酶（D3）活性的影响。
将 24 只 3 月龄 SHR 分为 4 组：假手术+安静组、假手术+训练组、MI+安静组和 MI+训练组。MI 通过冠状动脉永久性结扎。运动训练（跑步机）在 MI 后 96 小时开始，持续 4 周（~60%最大努力，每周 4 次，每次 40 分钟/天）。测量心脏功能（超声心动图）、硫氧还蛋白还原酶（TRx）、总羰基水平等氧化应激标志物和 D3 活性。采用广义估计方程，然后进行 Bonferroni 检验（p<0.05）。
MI 导致左心室质量增加（p=0.002），心输出量降低（22.0%，p=0.047），射血分数降低（41%，p=0.008），羰基水平升高（p=0.001）和 D3 活性升高（33%，p<0.001）。运动训练导致左心室质量降低，恢复心输出量（34%，p=0.048）和射血分数（20%，p=0.040），TRx 增加（85%，p=0.007），羰基水平降低（p<0.001）和 D3 活性降低（p<0.001）。
我们的短期运动训练有助于逆转 MI 对心脏功能的影响。这些益处似乎源自心脏组织中更有效的抗氧化反应和更低的 D3 活性。
