表达荧光标记的ATOX1的基因编辑人类胚胎干细胞的产生
Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1.
作者信息
Wen Meng-Hsuan, Xie Xihong, Tu Jian, Lee Dung-Fang, Chen Tai-Yen
机构信息
Department of Chemistry, University of Houston, Houston, Texas 77204, United States.
Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, United States; Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
出版信息
Stem Cell Res. 2019 Dec;41:101631. doi: 10.1016/j.scr.2019.101631. Epub 2019 Oct 16.
ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endogenous regulatory machinery. The fluorescence engineered hESC clone maintains characteristic stem cell features and can differentiate to all three germ layers, serving as a unique tool to dissect the role of ATOX1 in various cellular processes.
ATOX1是一种参与细胞内铜稳态、细胞增殖和肿瘤进展的铜伴侣蛋白。为了通过基于成像的方法研究ATOX1的生理相关分子机制，我们在H1人胚胎干细胞中对ATOX1进行基因改造，使其在内源调控机制下表达mCherry-ATOX1融合蛋白。荧光工程化的人胚胎干细胞克隆保持了特征性的干细胞特性，并且可以分化为所有三个胚层，是剖析ATOX1在各种细胞过程中作用的独特工具。
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