Sowers K R, Gunsalus R P
Department of Microbiology, University of California, Los Angeles 90024.
J Bacteriol. 1988 Oct;170(10):4979-82. doi: 10.1128/jb.170.10.4979-4982.1988.
Nine acetotrophic and three methylotrophic strains of methane-producing bacteria were screened for the presence of plasmid DNA. Plasmids were detected in three marine isolates, including Methanosarcina acetivorans. All three plasmids appeared to be similar based on size and restriction site analyses. The plasmid from M. acetivorans, designated pC2A, was approximately 5.1 kilobase pairs in size and was estimated to be present in a low copy number of six plasmids per genome. Multimers were also observed. A restriction map was constructed. The function of this plasmid is cryptic.
对9株产乙酸型和3株甲基营养型产甲烷细菌进行了质粒DNA检测。在3株海洋分离株中检测到了质粒，其中包括嗜乙酰甲烷八叠球菌。基于大小和限制酶切位点分析，所有这3种质粒似乎都很相似。嗜乙酰甲烷八叠球菌的质粒命名为pC2A，大小约为5.1千碱基对，估计每个基因组中以低拷贝数存在6个质粒。还观察到了多聚体。构建了限制酶切图谱。该质粒的功能未知。
