Meile L, Kiener A, Leisinger T
Mol Gen Genet. 1983;191(3):480-4. doi: 10.1007/BF00425766.
The archaebacterium Methanobacterium thermoautotrophicum Marburg (DSM 2133) was found to contain a plasmid (pME2001) in covalently closed circular form. It was isolated by CsCl gradient centrifugation of total DNA in the presence of ethidium bromide. Multimers up to the hexamer were observed upon agarose gel electrophoresis and electron microscopy of a purified plasmid preparation. A restriction map was constructed. The length of plasmid pME2001 was determined to be approximately 4,500 bp. Southern hybridization of plasmid DNA to DNA extracted from Methanobacterium thermoautotrophicum delta H (DSM1053) revealed the presence of a plasmid with homologous sequences in the delta H strain.
发现嗜热自养甲烷杆菌马尔堡菌株（DSM 2133）含有共价闭合环状形式的质粒（pME2001）。它是通过在溴化乙锭存在下对总DNA进行氯化铯梯度离心分离得到的。在纯化的质粒制剂的琼脂糖凝胶电泳和电子显微镜观察中观察到了直至六聚体的多聚体。构建了限制性图谱。确定质粒pME2001的长度约为4500 bp。质粒DNA与从嗜热自养甲烷杆菌δH菌株（DSM1053）提取的DNA进行Southern杂交，结果显示δH菌株中存在具有同源序列的质粒。
