Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and Automation, Shanghai University, Shanghai, 200444, China.
Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai, 200444, China.
Appl Biochem Biotechnol. 2020 Mar;190(3):1106-1123. doi: 10.1007/s12010-019-03147-2. Epub 2019 Nov 8.
Electrospinning is a promising method for preparing bionic vascular scaffolds. In particular, coaxial electrospinning can encapsulate polymer materials in biological materials and provide vascular scaffolds with good biomechanical properties. However, it is difficult to produce a stable Taylor cone during the coaxial electrospinning process. Moreover, glutaraldehyde cross-linked natural biomaterials are cytotoxic. To address these issues, a novel electrospinning process is proposed in this report. A non-ionic surfactant (Tween 80) was added to poly(lactic-co-glycolic acid) electrospinning solution and gelatin-collagen electrospinning solution, which prevented the interfacial effect of coaxial electrospinning due to different core/shell solutions. The as-prepared materials were then cross-linked with the non-toxic coupling agents N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide/N-hydroxysuccinimide (EDC/NHS). By comparing the biomechanical properties of EDC/NHS cross-linked vascular scaffold with glutaraldehyde vapor-cross-linked vascular scaffold, it was found that the fracture strain and biological performance of EDC/NHS cross-linked vascular scaffold were better than those of the glutaraldehyde cross-linked scaffold. Finally, a three-layer bionic vascular scaffold was prepared by the proposed electrospinning process. Biomechanical performance tests were carried out and the prepared scaffold was found to meet the requirements of tissue-engineered blood vessels. The research in this paper provides a useful reference for the preparation and optimization of vascular scaffolds.
静电纺丝是一种很有前途的仿生血管支架制备方法。特别是同轴静电纺丝可以将聚合物材料封装在生物材料中，为血管支架提供良好的生物力学性能。然而，在同轴静电纺丝过程中很难产生稳定的泰勒锥。此外，戊二醛交联的天然生物材料具有细胞毒性。针对这些问题，本报告提出了一种新的静电纺丝工艺。在聚乳酸-共-羟基乙酸静电纺丝溶液和明胶-胶原静电纺丝溶液中添加非离子表面活性剂（吐温 80），以防止由于不同的核/壳溶液而导致的同轴静电纺丝的界面效应。然后用非毒性偶联剂 N-(3-二甲氨基丙基)-N'-乙基碳二亚胺/N-羟基琥珀酰亚胺（EDC/NHS）对所制备的材料进行交联。通过比较 EDC/NHS 交联血管支架与戊二醛蒸汽交联血管支架的生物力学性能，发现 EDC/NHS 交联血管支架的断裂应变和生物性能优于戊二醛交联支架。最后，通过提出的静电纺丝工艺制备了三层仿生血管支架。进行了生物力学性能测试，发现所制备的支架符合组织工程血管的要求。本文的研究为血管支架的制备和优化提供了有益的参考。
