Bacteriology Department, University Hospital Dijon, PTB, BP, 37013, 21070, Dijon Cedex, France; UMR 6249, Chrono-Environnement, PTB, BP 37013, 21070, Dijon Cedex, France; School of Life and Environmental Sciences, The University of Sydney, NSW 2006, Australia.
Bacteriology Department, University Hospital Dijon, PTB, BP, 37013, 21070, Dijon Cedex, France; UMR 6249, Chrono-Environnement, PTB, BP 37013, 21070, Dijon Cedex, France.
Plasmid. 2020 Jan;107:102453. doi: 10.1016/j.plasmid.2019.102453. Epub 2019 Nov 6.
Several groups of integrative mobilizable elements (IMEs) that harbour a class 1 integron carrying antibiotic resistance genes have been found at the 3'-end of the chromosomal trmE gene. Here, a new IME, designated SGI0, was found in trmE in the sequenced and assembled genome of a French clinical, multiply antibiotic resistant Proteus mirabilis strain, Pm1LENAR. SGI0 shares the same gene content as the backbones of SGI1 and SGI2 (overall 97.6% and 97.7% nucleotide identity, respectively) but it lacks a class 1 integron. However, SGI0 is a mosaic made up of segments with >98.5% identity to SGI1 and SGI2 interspersed with segments sharing 74-95% identity indicating that further diverged backbone types exist and that recombination between them is occurring. The structure of SGI1-V, here re-named SGI-V, which lacks two SGI1 (S023 and S024) backbone genes and includes a group of additional genes in the backbone, was re-examined. In regions shared with SGI1, the backbones shared 97.3% overall identity with the differences distributed in patches with various levels of identity. The class 1 integron is also in a slightly different position with the target site duplication AAATT instead of ACTTG for SGI1 and variants, indicating that it was acquired independently. The Pm1LENAR resistance genes are in the chromosome, in Tn7 and an ISEcp1-mobilised segment.
已经在染色体 trmE 基因的 3'末端发现了几组长整合可移动元件（IMEs），这些元件携带着带有抗生素抗性基因的类 1 整合子。在这里，在法国临床、多抗生素耐药性奇异变形杆菌株 Pm1LENAR 的测序和组装基因组中，在 trmE 中发现了一个新的 IME，命名为 SGI0。SGI0 与 SGI1 和 SGI2 的骨架具有相同的基因组成（分别为 97.6%和 97.7%的核苷酸同一性），但它缺乏类 1 整合子。然而，SGI0 是一个由与 SGI1 和 SGI2 具有 >98.5%同一性的片段组成的嵌合体，其中穿插着具有 74-95%同一性的片段，表明存在进一步分化的骨架类型，并且它们之间正在发生重组。在这里重新命名为 SGI-V 的 SGI1-V 的结构，它缺少两个 SGI1（S023 和 S024）骨架基因，并在骨架中包含一组额外的基因，被重新检查。在与 SGI1 共享的区域中，骨架之间的总体同一性为 97.3%，差异分布在具有不同同一性水平的补丁中。类 1 整合子的位置也略有不同，靶位点重复为 AAATT 而不是 SGI1 和变体的 ACTTG，表明它是独立获得的。Pm1LENAR 抗性基因位于染色体、Tn7 和 ISEcp1 动员的片段中。
