Bouhours D, Bouhours J F
Unité 76 de l'Institut National de la Santé et de la Recherche Médicale, Centre National de Transfusion Sanguine, Paris, France.
J Biol Chem. 1988 Oct 25;263(30):15540-5.
The ganglioside composition of the epithelial cells of the small intestine was investigated in 15 strains of inbred rats. Most of these strains had GM3 as the only detectable ganglioside. In addition to GM3, small amounts of GD3 were found in four strains, AVN, BN, DA, and LE. The fatty acid content of the ceramide portion was composed of a large, although variable, percentage of 2-hydroxy fatty acids. The sphingoid base was always C18-4D-hydroxysphinganine. The highly prominent sialic acid was N-glycolylneuraminic acid (NeuGc) in most strains. However in two strains, Brown Norway (BN) and spontaneously hypertensive rats (SHR), NeuAc was the only sialic acid of the gangliosides of the intestinal epithelium. The analysis of the ganglioside composition of the epithelium of the small intestine of the first generation hybrids of SHR with DA and BN, respectively, demonstrated that the expressions of GM3 (NeuGc) and GD3 were genetically transmitted as dominant traits and that BN and SHR were likely to carry the same deficient gene that led to the expression of GM3(NeuAc) instead of GM3(NeuGc) in the small intestine. For comparison, the sialic acid composition of kidney gangliosides was analyzed in some strains. 21-23% of the kidney gangliosides was GM3(NeuGc) in all tested strains, including BN and SHR. Therefore, the ganglioside composition of the intestinal epithelium could vary in the rat species, and the defect of N-glycolylneuraminic acid was not only strain-specific but also occurred in a tissue-specific way among strains of inbred rats.
对15个近交系大鼠品系的小肠上皮细胞神经节苷脂组成进行了研究。这些品系中的大多数仅能检测到GM3这一种神经节苷脂。除GM3外，在四个品系（AVN、BN、DA和LE）中还发现了少量的GD3。神经酰胺部分的脂肪酸含量由很大比例（尽管存在变化）的2-羟基脂肪酸组成。鞘氨醇碱基始终为C18-4D-羟基鞘氨醇。在大多数品系中，高度突出的唾液酸是N-羟乙酰神经氨酸（NeuGc）。然而，在两个品系，即棕色挪威大鼠（BN）和自发性高血压大鼠（SHR）中，NeuAc是肠上皮神经节苷脂的唯一唾液酸。分别对SHR与DA和BN的第一代杂交种小肠上皮神经节苷脂组成进行分析，结果表明GM3（NeuGc）和GD3的表达作为显性性状进行遗传传递，并且BN和SHR可能携带相同的缺陷基因，该基因导致小肠中表达GM3（NeuAc）而非GM3（NeuGc）。为作比较，对一些品系的肾神经节苷脂唾液酸组成进行了分析。在所有测试品系（包括BN和SHR）中，21%-23%的肾神经节苷脂为GM3（NeuGc）。因此，大鼠物种中小肠上皮的神经节苷脂组成可能存在差异，并且N-羟乙酰神经氨酸的缺陷不仅具有品系特异性，而且在近交系大鼠品系中以组织特异性方式出现。
