Ando N, Yamakawa T
J Biochem. 1982 Mar;91(3):873-81. doi: 10.1093/oxfordjournals.jbchem.a133775.
Seven ganglioside species were isolated and purified from erythrocyte membranes of Japanese cats by DEAE-Sephadex and Iatrobeads column chromatographies. The structures of these gangliosides were determined as Gmi(NeuGc), Gm3(NeuAc), GM3(NeuGc), GD3(NeuGc), GD3(NeuGc comes from NeuAc), GT3(NeuGc), and another GM3 containing a sialic acid of unidentified nature. The occurrence of GT3 suggested the probable presence of a biosynthetic pathway of GM3 leads to GD3 leads to GT3 in erythropoietic cells of Japanese cats. The presence of GD3 having one penultimate N-glycolylneuraminic acid and one terminal N-acetylneuraminic acid, GD3(NeuGc comes from NeuAc) would indicate that this GD3 acts as an intermediate in a possible pathway from GM3(NeuGc) to GD3(NeuGc). Thin layer chromatographic patterns of total erythrocyte membrane gangliosides were compared among Japanese cats (n = 3), lions (n = 3), a serval and a racoon dog. The three species of felid showed similar patterns to each other and contained N-glycolylneuraminic acid as the major sialic acid. On the other hand, erythrocytes of racoon dog, a member of canidae, contained neither GD3 nor GT3, but only GM3.
通过DEAE - 葡聚糖和离子交换树脂柱色谱法从日本猫的红细胞膜中分离并纯化出七种神经节苷脂。这些神经节苷脂的结构被确定为Gmi(NeuGc)、Gm3(NeuAc)、GM3(NeuGc)、GD3(NeuGc)、GD3(NeuGc来自NeuAc)、GT3(NeuGc)，以及另一种含有性质不明唾液酸的GM3。GT3的存在表明在日本猫的红细胞生成细胞中可能存在一条从GM3到GD3再到GT3的生物合成途径。含有一个倒数第二个N - 羟乙酰神经氨酸和一个末端N - 乙酰神经氨酸的GD3，即GD3(NeuGc来自NeuAc)的存在表明该GD3可能是从GM3(NeuGc)到GD3(NeuGc)的可能途径中的一个中间产物。比较了日本猫（n = 3）、狮子（n = 3）、一只薮猫和一只貉的全红细胞膜神经节苷脂的薄层色谱图谱。三种猫科动物的图谱彼此相似，且以N - 羟乙酰神经氨酸作为主要唾液酸。另一方面，犬科动物貉的红细胞既不含GD3也不含GT3，仅含有GM3。
